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2025年8月11日 星期一
  2016, Vol. 24 Issue (12): 1820-1830    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
89份大麦遗传多样性分析及其网斑病抗性位点相关SSR标记筛选
孟亚雄1,张海娟2,马小乐2,王育才3,汪军成2,司二静1,任盼荣4,杨轲3,张京5,王化俊6
1. 甘肃农业大学农学院
2. 甘肃农业大学
3. 甘肃农业大学 甘肃省干旱生境作物学重点实验室
4. 甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室
5. 中国农业科学院 作科所
6. 甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与创新重点实验室
Genetic Diversity and Screening of SSR Markers Associated with Net Blotch Resistance in 89 Barley (Hordeum vulgare) Cultivars
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摘要 大麦网斑病(Pyrenophora teres)是大麦主要病害之一。近年来网斑病在我国大麦(Hordeum vulgare)产区发生并流行,网斑病之所以在我国各大麦产区发生并流行,主要是由于生产中缺乏抗网斑病品种。为分析大麦种质材料遗传多样性,筛选与大麦抗网斑病性状相关联的SSR标记,本研究利用70对品种间表现多态性的SSR标记对89个大麦品种(系)进行分析。结果显示,70个SSR标记共检测出302个等位变异,平均4.31个,变幅为2~8个;等位基因频率为0.141 2~0.916 7;基因多样性和遗传相似系数的变化范围分别是0.103 9~0.894 4和0.520~0.873;多态性信息含量(polymorphic information content, PIC)为0.098 5~0.884 6,平均为0.537。群体遗传结构分析表明,供试大麦亲本材料可分为2个亚群,群体遗传相似系数变幅为0.635~0.895。品种美41/I和美43/I的遗传相似系数最高,为0.895。根据一般线性模型(general linear model, GLM)分析,发现5个与大麦抗网斑病相关的标记,解释率在7.2%~22.4%之间,标记Bmac29和Bmag0613关联达到极显著水平(P<0.01),对表型变异的解释率为分别为10.7%和22.4%。本研究为大麦网斑病抗病育种提供了一定理论依据。
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作者相关文章
孟亚雄
张海娟
马小乐
王育才
汪军成
司二静
任盼荣
杨轲
张京
王化俊
关键词 大麦遗传多样性SSR大麦网斑病抗性关联分析    
Abstract:The barley (Hordeum vulgare) net blotch is one of the most usual barley diseases, caused by Pyrenophora teres. The barley net blotch is becoming so popular, the major reasons is that our country is short of germplasm resources of resisting barley net blotch. In order to increase the diversity of barley net blotch resistance in cultivated barley, the genetic diversity of barley was analysed, and the reaction to P. teres of 89 barley materials at seedling stage was evaluated. Eventually, identified SSR markers associated with resistance to barley net blotch in barley with an association mapping approach. The results showed that 89 barley entries were analyzed by using 70 pairs of SSR markers which were used to detect the genetic diversity among cultivars. A total of 302 alleles were detected, the average each pairs had 4.31 and the arrange of allele number was 2~8. The allele frequency varied from 0.141 2~0.916 7, the genetics diversity ranged from 0.103 9~0.894 4, and the genetics similarity ranged from 0.520~0.873. The polymorphism information content (PIC) value ranged from 0.098 5~0.884 6 with an average of 0.537. Barley entries were divided into 2 subgroups based on their population structure, group one had 22 cultivars, group two had 67 cultivars, the genetic similarity of the all group was from 0.635~0.895. The genetic similarity of MEI 41/I and MEI 43/I was the highest, it was 0.895. There was the large difference among barley materials, and disease index ranged from 31.67~96.32, and there were no immune or high resistant varieties, just 2 resistant-middle resistant (R-M) varities with the disease index of 31.67 and 33.56, one cultivar (ZD02732407) was moderate resistant (MR), disease index was 45.22%, and then there were 14 middle resistant-middle susceptible (MS-S) varieties, 50 middle susceptible-susceptible (MS-S) varieties and 7 high susceptible (S) varieties. The association analysis result showed that 5 markers were found to associate with barley net blotch resistance under general linear model (GLM) program, these QTLs were located on chromosomes 1H, 3H, 5H and 6H, and the rate of phenotypic variation explained ranged from 7.2%~22.4%. Bmac29 and Bmag0613 showed significant association with resistance to barley net blotch (P<0.01), which explained 10.7% and 22.4% of the phenotypic variation. This study demonstrates that AM is an effective technique for identifying and mapping QTL for barley net blotch resistance in a natural population and then this study also provides some helpful basis to barley net blotch resistant breeding.
Key wordsBarley    Genetic diversity    SSR    Barley net blotch resistance    Association analysis
收稿日期: 2016-06-15      出版日期: 2016-11-07
基金资助:大麦抗网斑病基因的遗传特性研究及精细定位;国家大麦青稞产业技术体系
通讯作者: 王化俊     E-mail: whuajun@yahoo.com
引用本文:   
孟亚雄 张海娟 马小乐 王育才 汪军成 司二静 任盼荣 杨轲 张京 王化俊. 89份大麦遗传多样性分析及其网斑病抗性位点相关SSR标记筛选[J]. , 2016, 24(12): 1820-1830.
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http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2016/V24/I12/1820
 
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