Abstract：The wall-associated kinase (WAK) is a unique class of receptor-like kinase (RLK), which plays an important role in regulating cell growth and defense against stress response. In this study, 27 CsWAK genes were identified from the tea plant (Camellia sinensis) genome using bioinformatics methods, which were mainly clustered on 2 chromosomes and 1 contig. These CsWAK were classified into 5 subgroups (Ⅰ~Ⅴ) based on the phylogenetic analysis of the protein sequences, and most members of the same subgroup had similar gene structures and conserved motifs. The transcriptome data analysis showed that most CsWAK were expressed primarily in tea leaves, followed by roots and stems. Most members of the CsWAK family had little difference in response to biotic/abiotic stresses such as hormone, low temperature, and high salt. The difference of most CsWAK gene expression were not significant, only several CsWAK gene expression had significant difference, with diversity and specificity. In addition, some differentially expressed CsWAK in response to cold treatment, PEG treatment and salt treatment were analyzed by qPCR. The results showed that under the stress of low-temperature, the selected 9 genes all showed an expression pattern that rose first and then fell, and most of the genes were significantly upregulated at low-temperature treatment for 3 h; under the stress of PEG, the expression level of the selected 9 genes was slightly different from the transcriptome data, but the expression trend was similar; and under NaCl treatment, the expression level of the selected 9 genes was significantly downregulated compared to that of untreated, among them, CsWAK14 was the most sensitive to salt stress. These results provide a reference for further analysis of the biological functions of CsWAK gene.
焦小雨, 吴琼, 刘丹丹, 孙明慧, 王文杰. 茶树细胞壁关联蛋白激酶基因家族的鉴定与表达分析[J]. 农业生物技术学报, 2023, 31(9): 1816-1831.
JIAO Xiao-Yu, WU Qiong, LIU Dan-Dan, SUN Ming-Hui, WANG Wen-Jie. Identification and Expression Analysis of the Wall-associated Kinase Gene Family in Camellia sinensis. 农业生物技术学报, 2023, 31(9): 1816-1831.
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