Identification of Lipoxygenase Family Genes in Maize (Zea mays) and Expression Analysis Under Fusarium graminearum Infection and MeJA Treatment
LI Wei1, ZHANG Wei-Yu1, LIU Ying-Zi2, MA Wen-Xiao1, LI Zi-Yuan1, ZHANG Kang1, XING Ji-Hong1, CAO Hong-Zhe1,*, DONG Jin-Gao1,*
1 State Key Laboratory of North China Crop Improvement and Regulation / Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding 071000, China; 2 Garden Management Centre of Hebei Province, Chengde 067000, China
Abstract：Lipoxygenase (LOX) is an important oxidoreductase in plants, which is widely involved in plant growth, development and response to biological and abiotic stresses. At present, the members of maize (Zea mays) LOX family and their roles in jasmonic acid (JA) signaling pathway are still not clear. In order to explore the function of LOX family genes in maize, bioinformatics methods were used to identify maize LOX family genes, and analyzed their phylogenetic evolution, conserved motifs, tissue expression specificity and expression patterns under biological and abiotic stresses. The expression of maize LOX family genes inoculated with Fusarium graminearum and induced by methyl jasmonate (MeJA) were detected by qRT-PCR. The results showed that maize genome contained 13 LOX family genes, which could be divided into 9-LOX, 13-LOX Type Ⅰ and 13-LOX Type Ⅱ, and all of them contained conservative lipoxygenase motif. The expression levels of ZmLOXs showed significant differences in different tissues or under different biological and abiotic stresses (P<0.05). After F. graminearum inoculation and MeJA treatment, the expression levels of 13 LOX family genes in maize were significantly up-regulated (P<0.05). These results suggested that maize LOX family genes might have important functions in maize growth and development and response to different biological and abiotic stresses. This study provides a reference for further understanding the function and mechanism of LOX family genes in maize.
李薇, 张玮煜, 刘英姿, 马文骁, 李紫媛, 张康, 邢继红, 曹宏哲, 董金皋. 玉米脂氧合酶家族基因的鉴定及禾谷镰刀菌侵染与茉莉酸甲酯处理下的表达分析[J]. 农业生物技术学报, 2023, 31(8): 1567-1577.
LI Wei, ZHANG Wei-Yu, LIU Ying-Zi, MA Wen-Xiao, LI Zi-Yuan, ZHANG Kang, XING Ji-Hong, CAO Hong-Zhe, DONG Jin-Gao. Identification of Lipoxygenase Family Genes in Maize (Zea mays) and Expression Analysis Under Fusarium graminearum Infection and MeJA Treatment. 农业生物技术学报, 2023, 31(8): 1567-1577.
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