Identification and Cold Stress Expression Analysis of CML Gene Family in Erianthus fulvus Based on Transcriptome
QIAN Zhen-Feng1, GU Shu-Jie1, ZHAO Xue-Ting1, RAO Xi-Bing1, ZENG Dan2, SHEN Qing-Qing2, ZHANG Rong-Qiong2, CHEN Shu-Ying2, HE Li-Lian1,2,*, LI Fu-Sheng1,2,*
1 College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China; 2 Sugarcane Research Institute, Yunnan Agricultural University, Kunming 650201, China
Abstract：Calmodulin-like (CML) is a calcium-binding protein in plant cells that is involved in signal transduction during growth, development, and stress. In order to explore the expression pattern of CML family genes in Erianthus fulvus under cold stress, 39 EfCML family genes were identified based on transcriptome data, and bioinformatics and expression analysis under cold stress were carried out. The analysis of the physicochemical properties of the proteins showed the number of amino acid residues, molecular weight, and isoelectric point of EfCMLs proteins was 59~312, 6.240 21~34.017 78 kD, and 3.99~11.14, respectively. The predicted results of subcellular localization showed that the 39 EfCMLs were located in the cytoplasm, cell membrane, and nucleus, respectively. Conservative domain analysis showed that EfCML family proteins contained 1~4 typical EF-hand domains. Motif analysis showed that EfCML family proteins contained 1~4 typical Ca2+ binding motifs. Phylogenetic tree analysis showed that EfCMLs proteins could be divided into 11 groups. Expression analysis of cold stress showed that low temperature could induce differential expression of EfCML family genes, with EfCML1 and EfCML2 were consistently up-regulated, and reached peak value at 72 h cold stress with 27 and 35 times higher than that of the control (P<0.01), showing the most active state. This study provides basic data for in-depth analysis of the cold stress regulation mechanism of E. fulvus CML genes.
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