Genome-wide Identification of LEA-2 Gene Family in Zea mays and ZmLEA53 Functional Preliminary Verification
WANG Guo-Rui1,2,*, LU Xiao-Min2,*, ZHANG Peng-Yu1,3, CAO Li-Ru2, GUO Jin-Sheng2, FU Jia-Xu1, WANG Tong-Chao1, WEI Li1,**
1 College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China; 2 Cereal Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 3 Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
Abstract：Late embriogenesis abundant protein-2 (LEA-2) gene family play important role in plant growth,development and abiotic stresses. In order to investigate the response of ZmLEA-2 family proteins to drought stress, the LEA-2 protein sequence in maize (Zea mays), Sorghum bicolor, and Arabidopsis thaliana were aligned, also analyzed the gene structure,conserved motifs and position on the chromosome. The collinearity of ZmLEA-2 gene family in maize species were analyzed. Collinearity analysis between the maize, sorghum and Arabidopsis thaliana were also performed. The expression pattern of ZmLEA-2 family genes were analyzed based on the transcriptome data, and the function of ZmLEA53 was preliminary verificated.The results showed that 81 ZmLEA-2 genes were unevenly distributed on 10 chromosomes; the phylogenetic tree analysis showed that 81 ZmLEA-2 genes were divided into 7 groups, and genes in the same group had similar gene structure and conserved motifs. 81 ZmLEA-2 genes exhibited different expression patterns. Under drought stress, the expression of ZmLEA53, ZmLEA7 and ZmLEA64 was up-regulated and decreased after rewatering. It was speculated that these genes were involved in the regulation of drought tolerance. qPCR results showed that the expression of ZmLEA53 was positively regulated by drought, salt and high temperature. Overexpression of ZmLEA53 could improve the survival rate of yeast (Saccharomyces) cells under drought (PEG), salt and high temperature stress, respectively. The results provide a basis for further functional identification of ZmLEA-2 gene family under drought stress.
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