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2025年4月6日 星期日
农业生物技术学报  2021, Vol. 29 Issue (8): 1485-1494    DOI: 10.3969/j.issn.1674-7968.2021.08.005
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
苎麻液泡膜质子焦磷酸酶基因BnVP1的克隆及镉胁迫下的表达分析
朱守晶1,2,*, 史文娟1
1 宜春学院 生命科学与资源环境学院,宜春 336000;
2 江西省作物生长发育调控重点实验室,宜春 336000
Cloning of the Vacuolar H+-pyrophosphatases Gene BnVP1 from Ramie (Boehmeria nivea) and Expression Analysis Under Cadmium Stress
ZHU Shou-Jing1,2,*, SHI Wen-Juan1
1 College of Life Science and Resources and Environment, Yichun University, Yichun 336000, China;
2 Jiangxi Key Laboratory of Regulation and Control of Crop Growth and Development, Yichun 336000, China
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摘要 镉是一种伴随工农业生产而进入环境的重金属污染物,对多种生物具有较强的毒性。液泡区隔化是植物应对镉胁迫的重要机制之一。液泡膜质子焦磷酸酶(vacuolar H+-pyrophosphatase, V-H+-PPase)是一种存在于液泡膜上的质子泵,在无机离子的跨膜运输中发挥重要作用。本研究在前期苎麻(Boehmeria nivea)镉胁迫转录组分析的基础上,采用cDNA末端快速扩增(rapid-amplification of cDNA ends, RACE)技术从苎麻中克隆到一个V-H+-PPase基因BnVP1 (GenBank No. MW029619)。BnVP1基因的ORF长度为2 292 bp,编码763个氨基酸。BnVP1蛋白为跨膜蛋白,包含15个跨膜区,属于H_PPase超家族,与碧桃(Prunus persica) PpVP1 (XP_007208066.1)、苹果(Malus domestica) MdVP1 (XP_008359855.1)、可可(Theobroma cacao) TcVP1 (XP_017977384.1)、陆地棉(Gossypium raimondii) GrVP1 (XP_012476518.1)等Ⅰ型V-H+-PPase基因编码蛋白的氨基酸序列相似性较高,介于90.04%~92.27%之间。BnVP1启动子中含有若干与非生物逆境相关的诱导元件。qRT-PCR分析表明,BnVP1的表达不存在组织特异性。在镉胁迫下,苎麻根部与叶片中的BnVP1的表达量迅速增加达到最大值,显著受镉胁迫诱导。本研究丰富了苎麻耐镉分子机制的研究内容,为BnVP1基因的耐镉机制研究提供了参考。
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朱守晶
史文娟
关键词 液泡膜质子焦磷酸酶(V-H+-PPase)苎麻克隆表达分析    
Abstract:Cadmium is a heavy metal pollutant with high toxicity to many organisms, which enters into the environment along with industrial and agricultural activities. Vacuolar sequestration is an important mechanism to coping with cadmium stress in plants. Vacuolar H+-pyrophosphatase (V-H+-PPase), a proton pump in vacuolar membrane of plant cells, plays important roles in the transmembrane transport of inorganic ions. In this study, a V-H+-PPase gene, BnVP1 (GenBank No. MW029619) from ramie (Boehmeria nivea) was isolated by the rapid amplification of cDNA ends (RACE) method based on the previous transcriptome analysis of ramie under cadmium stress. The open reading frame sequence of BnVP1 was 2 292 bp and encoded 763 amino acids. BnVP1 was a transmembrane protein belonging to H_PPase superfamily, which contained 15 transmembrane domains. BnVP1 shared high amino acid sequence similarity (90.04%~92.27%) with typeⅠ V-H+-PPase proteins from Prunus persica (XP_007208066.1), Malus domestica (XP_008359855.1), Theobroma cacao (XP_017977384.1) and Gossypium raimondii (XP_012476518.1). The promoter sequence of BnVP1 contained several stress-related cis-acting elements. qRT-PCR showed that the expression of BnVP1 had no tissue specificity. The expression of BnVP1 in the root and leaves of ramie increased rapidly to maximum under cadmium stress, which indicated that the expression of BnVP1 was significantly induced by cadmium. The study enriches the research content of the molecular mechanism of cadmium tolerance in ramie, and provides a reference for the study of cadmium tolerance mechanism of Bnvp1 gene.
Key wordsVacuolar H+-pyrophosphatase (V-H+-PPase)    Cadmium    Ramie    Clone    Expression analysis
收稿日期: 2020-11-20     
ZTFLH:  S563.1  
基金资助:江西省教育厅科学技术研究项目(GJJ190857)
通讯作者: * zhusj85@sina.com   
引用本文:   
朱守晶, 史文娟. 苎麻液泡膜质子焦磷酸酶基因BnVP1的克隆及镉胁迫下的表达分析[J]. 农业生物技术学报, 2021, 29(8): 1485-1494.
ZHU Shou-Jing, SHI Wen-Juan. Cloning of the Vacuolar H+-pyrophosphatases Gene BnVP1 from Ramie (Boehmeria nivea) and Expression Analysis Under Cadmium Stress. 农业生物技术学报, 2021, 29(8): 1485-1494.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2021.08.005     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2021/V29/I8/1485
 
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