Cloning and Expression Analysis of LEPR Gene in Xupu Goose (Anser cygnoides)
QU Xiang-Yong1,2,*, LIU Xu1,2, DENG Yu-Ying1,2, CHENG Hao1,2, GUO Song-Chang1,2, HE Chang-Qing1,2
1 College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2 Hunan Collaborative Innovation Center of Animal Production Safety, Changsha 410128, China
Abstract：Leptin receptor (LEPR), a member of the class Ⅰ cytokine receptor superfamily, plays a key role in feed intake, energy balance and reproduction in mammals. Xupu goose (Anser cygnoides), an excellent local goose breed in Hunan Province, has excellent fatty liver performance and high nutritional value. To investigate the expression of LEPR at different tissues of Xupu goose, in this study, the coding sequence of LEPR was cloned by RT-PCR, and the putative protein spatial structure of LEPR was analyzed by biology software. Additionally, the expression of LEPR in Xupu goose different tissues and the correlation between LEPR expressions of adipose tissues and liver weight, ratio of liver to body weight, and blood biochemical indices were tested by quantitative Real-time PCR (qRT-PCR). The results showed that the coding sequence of LEPR was 3 468 bp, encoding 1 155 amino acids, which had high homology with LEPR gene of other poultry. phylogenetic analysis showed that Xupu goose had the closest genetic relationship with Zhedong white goose, followed by duck (Anas platyrhynchos) and chicken (Gallus gallus), and had a distant genetic relationship with mammals. LEPR gene was expressed in eight tissues of Xupu goose, including testis, spleen, sebum, liver, abdominal fat, heart, leg muscle, and chest muscle. The expression of LEPR gene in adipose tissue was negatively correlated with liver weight and aspartate aminotransferase (AST) content. This study provides a candidate gene for the molecular genetic study of liver type goose, and further provides a basis for studying the biological function of LEPR gene.
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