Abstract：Seed-related traits are important agronomic traits of crops, which are of great significance to melon (Cucumis melo) genetics and breeding and related molecular biology research.In this study, the thin-skinned melon small seed line 'P5' was used as the female parent, and the thick-skinned melon big seed line 'P10' was used as the male parent, F1 and F2 populations were obtained, 450 F2 plants were used to carry out genetic analysis of seed related traits. The results showed that the segregation ratio of seed shape and seed coat color was consistent with 3∶1 (χ2=0.26, 0.07). Both of them were quality traits controlled by a pair of alleles. The results of F2 population distribution showed that seed length, seed width and 100-seed weight showed unimodal normal distribution, which were quantitative traits. The 127 F2 individual plants were used to carry out specific-locus amplified fragment sequencing (SLAF-seq) technique and construct a genetic map of high saturation melon, and preliminary QTL analysis of seed-related characters was carried out. The results showed that a melon genetic map containing 12 linkage groups was constructed, and 3 716 markers were obtained, the total map distance was 1 356.49 cM, and the average genetic distance between markers was 0.37 cM. The locus SS3.1 controlling melon seed shape (SS) was located between Marker555072 and Marker555263 on melon chromosome 3, and the distance from the linkage markers on both sides was 83.91 and 84.34 cM, respectively, with a contribution rate of 26.45%. The locus SC12.1 controlling melon seed color (SC) was located between Marker1467315 and Marker1479935 on melon chromosome 12, and the distance from the linkage markers on both sides was 62.60 and 63.44 cM, respectively, and the contribution rate was 14.07%. Two QTL loci (HSW6.1 and HSW7.1) were detected to control the 100-seed weight of muskmelon seeds. HSW6.1 is located between Marker795158 and Marker795692 on chromosome 6, and the distances from the linkage markers on both sides are 17.67 and 21.03 cM, respectively, with a contribution rate of 13.97%. HSW7.1 is located between Marker854935 and Marker857435 on chromosome 7, and the genetic distances to the linkage markers on both sides are 3.17 and 5.45 cM, respectively, with a contribution rate of 9.76%. A QTL locus (SL3.1) controlling the seed length of muskmelon was detected, which was located between Marker554875 and Marker554952 of melon chromosome 3, The genetic distances between the locus and the linked markers on both sides are 82.65 and 83.49 cM, respectively. The contribution rate is 27.43%. Two QTL loci (SW3.1 and SW12.1) were detected to control the seed width of melon. SW3.1 is located between Marker554875 and Marker554924 on chromosome 3. The genetic distances to the linkage markers on both sides are 82.65 and 83.07 cM, respectively, with a contribution rate of 16.03%. SW12.1 is located between Marker1411425 and Marker1411798 on chromosome 12, and the genetic distances to the linkage markers on both sides are 29.83 and 29.83 cM, respectively, with a contribution rate of 12.88%. The results provide a theoretical basis for further cloning gene mining and functional analysis of melon seed related traits.
李俊凤, 王岭, 戴冬洋, 才羿, 杨丽敏, 王超, 盛云燕, 田丽美. 基于SLAF的甜瓜SNP遗传连锁图谱构建和种子性状QTL定位[J]. 农业生物技术学报, 2022, 30(4): 656-666.
LI Jun-Feng, WANG Ling, DAI Dong-Yang, CAI Yi, YANG Li-Min, WANG Chao, SHENG Yun-Yan, TIAN Li-Mei. Construction of SNP Genetic Linkage Map and QTL Mapping of Seed Traits in Melon (Cucumis melo) Based on SLAF. 农业生物技术学报, 2022, 30(4): 656-666.
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