Construction of Glaesserella parasuis ptsG Mutant Strain and Analysis of Its Partial Biological Characteristics
YAN Xue-Feng1, XIAO Wu-Dian2, GU Cong-Wei2, HE Man-Li2, ZHAO Ming-De2, HE Lyu-Qin2,*
1 School of Physical Education, Southwest Medical University, Luzhou 646000, China; 2 Laboratory Animal Center, Department of Science and Technology, Southwest Medical University, Luzhou 646000, China
Abstract：In the glucose-specific phosphotransferase (PTSGlc) system, glucose-specific enzyme ⅡBC (EⅡBCGlc) has a comprehensive effect on the metabolism of bacteria, but of which the function is still unclear in Glaesserella parasuis. In this study, EⅡBCGlc protein coding gene (ptsG) mutant and comlementary strain were constructed by natural transformation method. The growth status of wild strain SC1401 and ptsG mutant strains in the trypton soy broth (TSB)+glucose medium were compared in this study. Moreover, the bacterial morphology, biofilm formation, tolerance to oxygen pressure and virulence to mice (Mus musculus) of the SC1401 and ptsG mutant strain were measured. The results showed that the ptsG mutant strain could take in more reducing sugars for its own growth. Through transmission electron microscopy that compared with the wild strain, ptsG mutant strain had more high electron density areas inside the bacteria. In addition, the formation of biofilm and the tolerance to hydrogen peroxide were decreased in ptsG mutant strain. But there was no significant difference between SC1401 and ptsG mutant strains on mice. This study found that the ptsG gene played an important role in the formation of biofilm and oxidative stress tolerance, which will lay a foundation for the study of pathogenic mechanism in Glaesserella parasuis.
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