Construction of An Activation of Toxin 1 Gene C(ΔapxIC) Mutant of Actinobacillus pleuropneumoniae Gomphosised Outer Membrane Protein P2 Gene(ompP2) of Haemophilus parasuis
Abstract：Actinobacillus pleuropneumoniae (APP) and Haemophilus parasuis (Hps) are the bacteria of causing porcine disease. In this study, We constructed two recombinant vectores pBOSKΔIC-1 and pBOSKΔIC/ompP2 using APP5 (SW1) strain as a template, and the two recombinant vectores were used for plus-minus screening system with the basis of Kanr and sacB. The mutant strain, named SW1 Δ apxIC/ompP2, was obtained, which was deleted toxin I gene C (apxIC) in SW1 and gomphosised outer membrane protein P2 Hps gene (ompP2). The mutant missed a size of 475 bp apxIC gene but was inserted a size of 1107 bp Hps ompP2 gene compared with the parental strain SW1. No reverse mutation of apxIC gene was observed during after 10 generations, while Hps ompP2 gene still had a good genetic stability in vitro, the mutant strain had no significant difference of he basic growth characteristics with the parental strain (SW1). The results confirmed that an ΔapxIC mutant of A. pleuropneumoniae gomphosised ompP2 gene of H. parasuis was successfully constructed. and it offered a foundation for developing a new bivalent vaccine of APP and Hps
