Abstract:The total 48 Haemophilus parasuis strains isolated from Guangdong province in 2007-2008 were serotyped by Indirect Haemagglutination Assay, and respectively belonged to 7 serotypes, 5 serotype with 23.68%. Furthermore, the genotypes and phylogenetic relationship of all isolates were analysed by PHYLIP-3.68 after Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), the results indicated that the 48 isolates distributed to 31 genotypes among 8 clusters, especially B cluster with 66.67%. The findings suggested that the genetic polymorphism and serotypes of H.parasuis parasuis isolates are diverse in Guangdong province, and ERIC-PCR is an effective method of researching molecular epidemiology of animal infective diseases.