Abstract：Abstract Mycoplasma bovis (Mb) is an important pathogenic mycoplasma of cattle (Bos taurus). The biofilm formation of Mb can cause persistent infection in host cells and cause great economic loss to cattle industry. Therefore, the purpose of this study is to screen a dominant strain of Mb biofilm formation by identifying the biofilm formation ability of different Mb strains, and then optimize the cultural conditions on biofilm formation of Mb. First of all, the ability of biofilm formation of Mb PG45 strain, Hubei (HB) strain, Wuwei (WW) strain, Dingxi (DX) strain, and Lintao (LT) strain were identified by polystyrene microtiter plate assay, and then the strain with strong biofilm forming ability was used as the model strain to optimize the culture conditions of Mb biofilm formation. The results showed that the ability of biofilm formation was different among different strains of Mb, among which the biofilm formation ability of LT strain was the strongest, the next was HB strain. And the biofilm formation ability of PG45 strain, WW strain and DX strain was the weakest. The optimal cultural conditions for biofilm formation of Mb was that the Mb cultures (1.7E+08 CFU/mL) were inoculated at a dilution of 1∶10 into the Eaton medium containing 0.01 mg/mL fish sperm DNA, 20% serum of horse, 1% glucose and final pH 8.5, and then incubated at 37 ℃, with 5% CO2 for 72 h. Under the optimal cultural conditions, the biofilm formation ability of LT strain was sound. In this study, a dominant strain of Mb biofilm formation was obtained, and a stable and obvious biofilm was formed by optimizing the cultural conditions, which will provide excellent biomaterials for biofilm related study of Mb.
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