Screening Differentially-expressed Genes of Haemophilus parasuis from the Selective Capture of Transcribed Sequences (SCOTS) by Reverse Dot-blot Hybridization
Abstract：A reverse Dot-blot hybridization (RDH) method was established for screening the differentially-expressed genes of Haemophilus parasuis in vivo and in vitro from the selective capture of transcribed sequences (SCOTS) pools by optimizing the hybridization conditions including the kinds and concentration of target DNA, probe concentration, and UV irradiation time. Six samples were verified to be differentially-expressed genes in vivo of H. parasuis from ten samples of cDNA libraries (SCOTS pools) by RDH. The results showed that SCOTS was an effective approach to establish cDNA libraries containing differentially-expressed genes and RDH could be used to effectively pick up the differentially-expressed genes from the SCOTS pools.
