Effects of Propionate on Differentiation of Bovine (Bos taurus)Intramuscular Preadipocytes
WAN Rong1,*, ZHOU Zhen-Ming2, MENG Qing-Xiang2
1 College of Agriculture and Food Engineering, Baise University, Baise 533000, China; 2 College of Animal Science and Technology / Beef Cattle Research Center, China Agricultural University, Beijing 100193, China
Abstract：Intramuscular fat is an important indicatior of beef quality, and preadipocyte differentiation is crucial for the formation of marbled beef. The purpose of this study was to explore the effect of propionic acid on the differentiation of intramuscular preadipocytes in bovine, and to analyze the signal mechanism of intramuscular preadipocyte differentiation from the cellular and molecular level. Bovine intramuscular preadipocytes were isolated by CollagenaseⅠunder sterile conditions, differentiation medium with different combinations of different doses of propionate (1, 3 and 6 mmol/L), 0 µg/mL insulin and 0.25 µmol/L dexamethasonethe were designed to induce the differentiation of bovine intramuscular preadipocytes for 6 d. The results of determination of intracytoplasmic lipid contents and qRT-PCR analysis showed that, propionate significantly enhanced the intracytoplasmic lipid contents of bovine intramuscular preadipocytes in a dose dependent manner (P<0.05), and 1, 3 and 6 mmol/L significantly up-regulated the expression of PPARγ and C/EBPα mRNA of bovine intramuscular preadipocytes (P<0.05). The combination of propionate, insulin, and dexamethasone resulted in the greatest intracytoplasmic lipid content, whereas the removal of dexamethasone, insulin or propionate reduced intracytoplasmic lipid content by 85%, 77% and 68% respectively compared with the combination of propionate, insulin, and dexamethasone (P<0.05). Furthermore, the expression level of PPARγ and C/EBPα mRNA were decreased significantly (P<0.05). These results indicated that propionate may stimulate the differentiation of bovine intramuscular preadipocytes by acting as the first messenger of hormones (such as insulin or dexamethasone), and acting as a signal molecule through signal transduction mechanism. This study provides basic data for elucidating the deposition mechanism of intramuscular fat in beef cattle and realizing the nutritional regulation mode to produce high quality beef.
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