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2025年8月20日 星期三
农业生物技术学报  2019, Vol. 27 Issue (4): 684-691    DOI: 10.3969/j.issn.1674-7968.2019.04.012
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
FecB突变对小尾寒羊卵泡发育及AMHAMHR2基因表达的影响
陶林1, *, 郭晓飞2, *, 文禹粱1, 狄冉1, 刘秋月1, 胡文萍1, 张效生2, 张金龙2, 王翔宇1*, *, 储明星1*, *
1 中国农业科学院 北京畜牧兽医研究所,农业部动物遗传育种与繁殖重点实验室,北京 100193;
2 天津市畜牧兽医研究所,天津 300381
Effects of FecB Mutation on Follicular Development and Expression of AMH and AMHR2 Genes in Small Tail Han Sheep (Ovis aries)
TAO Lin1, *, GUO Xiao-Fei2, *, WEN Yu-Liang1, DI Ran1, LIU Qiu-Yue1, HU Wen-Ping1, ZHANG Xiao-Sheng2, ZHANG Jin-Long2, WANG Xiang-Yu1, **, CHU Ming-Xing1, **
1 Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2 Tianjin Institute of Animal Sciences, Tianjin 300381, China
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摘要 小尾寒羊(Ovis aries)是我国拥有高繁殖力的优良品种,其骨形态发生蛋白受体1B(bone morphogenetic protein receptor 1B, BMPR1B)基因发生FecB突变能提高排卵数和产羔数,但具体机制尚不清楚。本研究旨在探讨小尾寒羊不同FecB基因型(突变纯合型BB和野生型++)对卵泡数量和直径以及抗苗勒氏激素基因(anti-Müllerian hormone, AMH)和抗苗勒氏激素型受体基因(anti-Müllerian hormone receptor , AMHR2)表达模式的影响。基于TaqMan探针分型技术,运用同期发情方法,统计卵泡期++型(n=21)和BB型(n=16)直径在3 mm以上的卵泡数量和直径。利用qRT-PCR技术检测BB型小尾寒羊(n=3)卵泡期14种组织(下丘脑, 垂体, 卵巢, 心脏, 肝脏, 脾脏, 肺脏, 肾脏, 大脑, 肾上腺, 小肠, 输卵管, 子宫, 甲状腺)以及BB型和++型小尾寒羊黄体期和卵泡期(n=3, 3, 3, 3)下丘脑-垂体-卵巢性腺轴中AMHAMHR2的表达量。结果显示,FecB突变极显著增加了卵泡数量(P<0.01)和降低了卵泡直径(P<0.01)。AMHAMHR2在BB型小尾寒羊卵泡期各组织中均有表达,其中下丘脑-垂体-卵巢性腺轴中两者均高表达,卵巢中AMH表达量最高,肾上腺中AMHR2表达量最高。AMH在不同发育阶段不同FecB基因型小尾寒羊下丘脑-垂体-卵巢性腺轴的表达差异不显著(P>0.05)。AMHR2在卵巢中表达量无显著差异(P>0.05),在垂体中表达量BB型极显著高于++型(P<0.01),卵泡发育阶段和基因型对下丘脑AMHR2的表达量存在极显著影响(P<0.01)。综上表明,FecB突变可以引起小尾寒羊卵泡发育和AMHR2表达时空的改变,AMHR2在下丘脑-垂体-卵巢性腺轴系统的特定表达可能参与FecB突变调节卵泡发育,这为我国高繁殖力肉羊育种提供了新的理论依据。
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陶林
郭晓飞
文禹粱
狄冉
刘秋月
胡文萍
张效生
张金龙
王翔宇
储明星
关键词 小尾寒羊FecB突变卵泡抗苗勒氏激素基因(AMH)抗苗勒氏激素型受体基因(AMHR2)    
Abstract:As an excellent domestic breed with fecundity, Small Tail Han sheep (Ovis aries) could get high ovulation rate and little size as a result of A476G mutation in bone morphogenetic protein receptor 1B (BMPR1B) gene (FecB mutation), under which the mechanism remains to poorly understand. The objective of this study was to investigate the effects on the number and diameter of follicle and the expression patterns of anti-Müllerian hormone (AMH) gene and its receptor (anti-Müllerian hormone receptor , AMHR2) gene in Small Tail Han sheep with or without FecB mutation (mutant homozygous BB and wide type ++, respectively). Based on TaqMan probe genotyping and estrus synchronization, the number and diameter of follicle over 3 mm from ++ (n=21) and BB ewes (n=16) were recorded. qRT-PCR technology was applied to detect the expression of AMH and AMHR2 genes in 14 tissues (hypothalamus, pituitary, ovary, heart, liver, spleen, lung, kidney, brain, adrenal gland, small intestine, oviduct, uterus, thyroid) from 3 BB ewes during follicular phase and 3 tissues (hypothalamus, pituitary and ovary) from BB and ++ ewes during luteal phase and follicular phase (n=3, 3, 3, 3, respectively). The results revealed that FecB mutation had a highly significant increase in number of follicle (P<0.01) and a highly remarkable decrease in follicular diameter (P<0.01). Two genes were expressed in 14 tissues of BB ewes during follicular phase, wherein both genes expressed highly in hypothalamus-pituitary-ovary gonad axis as well as AMH gene expressed highest in ovary and AMHR2 gene in adrenal gland. The expression of AMH gene in hypothalamus-pituitary-ovary gonad axis between BB and ++ animals during different phases of follicular development were not significantly different (P>0.05). There was no significant difference for the expression of AMHR2 gene in ovary between 2 categories of animals during different phases of follicular development (P>0.05). The expression of AMHR2 gene in pituitary of BB ewes was higher than ++ contemporaries (P<0.01). Stage of follicular development and FecB genotype had a highly significant effect on expression of AMHR2 gene in hypothalamus (P<0.01). A conclusion can be drew that FecB mutation caused the change of follicular development and expression of AMHR2 gene in time and space in Small Tail Han sheep and the special expression of AMHR2 gene in hypothalamus-pituitary-ovary gonad axis system may be involved in the regulation that FecB mutation alters follicular development, which would give novel theoretical basis to multiparous meat sheep breeding in our country.
Key wordsSmall Tail Han sheep    FecB mutation    Follicle    Anti-Müllerian hormone (AMH) gene    Anti-Müllerian hormone receptor (AMHR2) gene
收稿日期: 2018-10-07     
ZTFLH:  S826  
  S826.8+9  
基金资助:国家自然科学基金(No. 31772580)、国家肉羊产业技术体系专项(No. CARS-38)、中央级公益性科研院所基本科研业务费专项(No.Y2017JC24)、中国农业科学院科技创新工程(No. ASTIP-IAS13)和天津市农业科技成果转化与推广项目(No. 201704020)
通讯作者: wangxiangyu@caas.cn;mxchu@263.net   
引用本文:   
陶林, 郭晓飞, 文禹粱, 狄冉, 刘秋月, 胡文萍, 张效生, 张金龙, 王翔宇, 储明星. FecB突变对小尾寒羊卵泡发育及AMHAMHR2基因表达的影响[J]. 农业生物技术学报, 2019, 27(4): 684-691.
TAO Lin, GUO Xiao-Fei, WEN Yu-Liang, DI Ran, LIU Qiu-Yue, HU Wen-Ping, ZHANG Xiao-Sheng, ZHANG Jin-Long, WANG Xiang-Yu, CHU Ming-Xing. Effects of FecB Mutation on Follicular Development and Expression of AMH and AMHR2 Genes in Small Tail Han Sheep (Ovis aries). 农业生物技术学报, 2019, 27(4): 684-691.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.04.012     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I4/684
 
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