Identification of Specific miRNA in Skin Follicles of Small Tail Han Sheep (Ovis aries) at Different Developmental Stages
HE Yu-Long1, 2, TAO Yu1, LI Yong2, YANG Yi2, ZHOU Xue-Zhang2, WU Yue-Hong1, 2, *
1 College of Life Science, Zhejiang Science and Technology University, Hangzhou 310018, China; 2 Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China
Abstract：Small Tail Han sheep (Ovis aries) is a sheep breed with both excellent meat and fur in China, and famous for its fast growing speed and high lambing rate. microRNA (miRNA) is post-transcriptional regulator factor which is involved in the regulation of development of skin and hair follicles in various animals. In the present study, in order to illustrate this regulation in Small Tail Han sheep, the miRNA expression profiles of the skin and hair follicles during adult (XWHY_1), postnatal 30 d (XWHY_2) and newborn (XWHY_3) were established and analyzed by using high throughput sequencing technology. The results showed that total 1 062 miRNAs, including 160 known miRNAs and 902 new miRNAs, were identified in these 3 developmental periods. By statistical analysis of these miRNAs, it was found that there were 69, 127, and 73 specifically expressed miRNAs in XYHY_1, XYHY_2 and XYHY_3, respectively. Total 666 miRNAs were commonly expressed in the 3 periods. Statistical analysis of differential expression of miRNAs was conducted by Fisher exact test and Chi-square test, and meet the condition of |log2(Fold_Change, FC)|≥1 and P≤0.05. By comparing XWHY_1, XWHY_2 and XWHY_3, 339 differentially expressed microRNAs were screened, 226 differentially expressed microRNAs were obtained after removing the repetition, and 2 645 target genes were predicted. Differentially expressed miRNAs were enriched by Kyoto encyclopedia of genes and genomes (KEGG) Pathway, results showed that 2 645 target genes were annotated to 181 signaling pathways. Differentially expressed of miRNAs was enriched by gene ontology (GO) and showed to be mainly participated in transcriptional regulation, formation of cell membrane and ion binding. The present study provides basic data to analyze the molecular mechanism of fur/velvet formation at miRNA level, and also provides a new thought to improve the yield and quality of wool/velvet of Small Tail Han sheep by means of manual regulation while maintaining its existing excellent quality.
何玉龙, 陶宇, 李勇, 杨易, 周学章, 吴月红. 不同发育阶段小尾寒羊皮肤毛囊特异性miRNA的鉴定[J]. 农业生物技术学报, 2019, 27(1): 89-96.
HE Yu-Long, TAO Yu, LI Yong, YANG Yi, ZHOU Xue-Zhang, WU Yue-Hong. Identification of Specific miRNA in Skin Follicles of Small Tail Han Sheep (Ovis aries) at Different Developmental Stages. 农业生物技术学报, 2019, 27(1): 89-96.
[1] 陈富强, 张小丽, 李发弟, 等. 2016. TLR4基因在小尾寒羊乳房炎乳腺组织中的表达[J]. 农业生物技术学报, 24(3): 349-356. (Chen F Q, Zhang X L, Li F D, et al.2016. The expression of TLR4 gene in mastitis breast tissue of Small Tail Han Sheep (Ovis aries)[J]. Journal of Agricultural Biotechnology, 24(3): 349-356.) [2] 赫晓燕, 郝欢庆, 刘丹丹, 等. 2010. 青年羊驼耳部和背部皮肤组织中miRNA差异表达研究[J]. 中国生物化学与分子生物学报, 26(11): 1016-1022. (He X Y, He H Q, Liu D D, et al.2010. Difference of microRNA expression in the ear and back skin of young Alpaca (Lama acos)[J]. Chinese Journal of Biochemistry and Molecular Biology, 26(11): 1016-1022.) [3] 姬改革, 束婧婷, 单艳菊, 等. 2018. 基于表达谱芯片筛选鸡不同部位皮肤组织差异表达基因[J]. 畜牧兽医学报, 49(1): 36-45. (Ji G G, Shu J T, Shan Y Jet al.2018. Identification of differentially expressed genes between different positions of chicken skin based on gene expression microarray[J]. Acta Veterinaria et Zootechnica Sinica, 49(1): 36-45.) [4] 江倩. 2015. miR-let7a在辽宁绒山羊毛囊发育周期中的差异表达及其靶基因功能鉴定[D]. 硕士学位论文, 吉林大学, 导师: 张巧灵, pp. 17-25. (Jiang Q.2015. Differential expression of miR-let7a in hair follicle cycle of Liaoning cashmere goats and identification of its tagetes[D]. Thesis for M.S., Ji Lin University, Supervisor: Zhang Q L, pp. 17-25.) [5] 李桂菊. 2016. 小尾寒羊被毛特征与繁殖率相关分析[J]. 现代农业科技, (2): 266-267. (Li G J. 2016. Analysis on relation between the characteristics of clothing hair and the breeding rate of Small Tailed Sheep[J]. Modern Agricultural Science and Technology, (2): 266-267.) [6] 李思敏. 1999. 小尾寒羊与滩羊裘用性能的比较[J]. 中国畜牧杂志, 35(2): 41-42. (Li S M.1999. Comparison of the performance of Small Tail Han sheep and Tan sheep[J]. Chinese Journal of Animal Science, 35(2): 41-42.) [7] 刘丹丹, 赫晓燕, 郝欢庆. 2010. MicroRNAs在动物皮肤和毛发发育中的调控作用[J]. 中国生物化学与分子生物学报, 26(9): 802-808. (Liu D D, He X Y, Hao H Q.2010. The regulatory role of microRNAs in the development of animal skin and hair[J]. Chinese Journal of Biochemistry and Molecular Biology, 26(9): 802-808.) [8] 宋伸, 杨敏, 蒋琳, 等. 2017. 基于高通量测序技术的山羊遗传多样性及其生产性状研究进展[J]. 中国畜牧兽医, 44(10): 2965-2972. (Song S, Yang M, Jiang L, et al.2017. Research progress on goat genetic diversity and production traits based on high-throughput sequencing technology[J]. China Animal Husbandry & Veterinary Medicine, 44(10): 2965-2972.) [9] 翟博, 张明新, 王春昕, 等. 2015. 高通量测序法检测新吉细毛羊和小尾寒羊miRNA差异表达[C]. 2015年全国养羊生产与学术研讨会. 中国畜牧兽医学会, 北京, pp. 26.(Zhai B,Zhang M X, Wang C X, et al. 2015. Differential expression of miRNA in new fine wool sheep and Small Tail Han sheep by high throughput sequencing[C]. 2015 National Symposium on Sheep Production and Symposium, Chinese Animal Husbandry and Veterinary Association, Beijing, pp. 26.) [10] 张立春, 孙福亮, 金海国, 等. 2017. 小尾寒羊与新吉细毛羊羊毛及毛囊性状比较研究[J]. 中国畜牧杂志, 53(9): 52-56. (Zhang L C, Sun F L, Jin H G, et al.2017. Comparative research on wool and wool follicles between Small Tail Han Sheep and Xinji fine wool sheep[J]. Chinese Journal of Animal Science, 53(9):52-56.) [11] Barozai M Y.2012. The novel 172 sheep (Ovis aries) microRNAs and their targets[J]. Molecular Biology Reports, 39(5): 6259-6266. [12] Du C D, Isaacs K, Moore G P.1993. Distribution of acidic and basic fibroblast growth factors in ovine skin during follicle morphogenesis[J]. Journal of Cell Science, 105(3): 667-674. [13] Gao W, Sun W, Yin J, et al.2017. Screening candidate microRNAs (miRNAs) in different lambskin hair follicles in Hu sheep[J]. PLoS One, 12(5): e0176532. [14] Grose R, Dickson C.2005. Fibroblast growth factor signaling in tumorigenesis[J]. Cytokine & Growth Factor Reviews, 16(2): 179-186. [15] Li J, Qu H, Jiang H, et al.2016. Transcriptome-wide comparative analysis of microrna profiles in the telogen skins of Liaoning Cashmere Goats (Capra hircus) and Fine-Wool Sheep (Ovis aries) by Solexa deep sequencing[J]. DNA & Cell Biology, 35(11): 696-705. [16] Nakayama F, Hagiwara A, Kimura M, et al.2009. Evaluation of radiation-induced hair follicle apoptosis in mice and the preventive effects of fibroblast growth factor-1[J]. Experimental Dermatology, 18(10): 889-892. [17] Powers C J, Mcleskey S W, Wellstein A.2000. Fibroblast growth factors, their receptors and signaling[J]. Endocrine Related Cancer, 7(3): 165-197. [18] Presta M, Dell'Era P, Mitola S, et al.2005. Fibroblast growth factor/fibroblast growth factor receptor system in angiogenesis[J]. Cytokine & Growth Factor Reviews, 16(2): 159-178. [19] Sheng X, Song X, Yu Y, et al.2011. Characterization of microRNAs from sheep (Ovis aries) using computational and experimental analyses[J]. Molecular Biology Reports, 38(5): 3161-3171. [20] Wen G Z, Jiang H W, Jin Q L, et al.2007. A subset of skin-expressed microRNAs with possible roles in goat and sheep hair growth based on expression profiling of mammalian microRNAs[J]. Omics: A Journal of Integrative Biology, 11(4): 385-396.
