Cloning, Structure and Expression Analysis of ADSL Gene in Xupu Goose (Anser cygnoides)
LIU Yao-Wen1,2, LIU Xu1,2, QU Xiang-Yong2*, GUO Song-Chang1,2, HE Chang-Qing1,2, JIANG Juan1,2
1 College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2 Hunan Collaborative Innovation Center of Animal Production Safety, Changsha 410128, China
Abstract：The adenylsuccinate lyase (ADSL) belongs to a member of the aspartase /fumarate superfamily family, and plays an important role in the initial synthesis of purine nucleotides and purine nucleotide cycle as well as improving muscle flavor. This study was to clone the cDNA full length of ADSL gene (GenBank No. MK161097) of Xupu goose (Anser cygnoides) by rapid amplification of cDNA ends (RACE), and analyze the ADSL mRNA level in different tissues/organs at sexual age (80 d), the nucleic acid and protein sequences by ADSL conducted by bioinformatics analysis. The result showed that the full length of ADSL cDNA sequence was 1 496 bp, containing a 202 bp 5'UTR, a 31 bp 3' UTR and a 1 263 bp ORF, encoding a protein of 420 amino acid, no signal peptide sites, non-secreted protein. The gene mainly found in cytoplasm by subcellular localization, and was an unstable hydrophilic protein. Sequence analysis revealed that the ADSL amino acid sequence of the Xupu goose was similar with those of the eastern Zhejiang White goose (99.68%), duck (Anas platyrhnchos, 88.35%), chicken (Gallus gallus, 85.64%), pigceon (Columba livia, 82.24%). Based on the relationship of ADSL to build construction system and showed that Xupu goose had the closest phylogenetic relationship to the eastern Zhejiang White goose, followed by the close relationship between duck and chicken, and the distant relationship with mammals. The qRT-PCR results showed that ADSL gene was expressed in different tissues, and the mRNA level of ADSL from high to low were breast muscles, leg muscle, liver, sebum and abdominal fat. The expression of ADSL in breast muscle was significantly higher than that of sebum and abdominal fat (P<0.05). These results provide a reference for further study on the mechanism of the ADSL gene in the goose meat quality.
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