Expression pattern Analysis of Peroxidase Family Genes in Fusarium graminearum
LIU Jian-Hu*, ZHANG Kang*, ZANG Jin-Ping, CAO Hong-Zhe, ZHANG Jing, XING Ji-Hong**, DONG Jin-Gao**
College of Life Sciences, Hebei Agricultural University/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/Mycotoxin and Molecular Plant Pathology Laboratory of Hebei Agricultural University, Baoding 071000, China
Abstract：As a main component of the anti-oxidant defense system of pathogenic fungi, peroxidase can remove reactive oxygen species (ROS) derived from plant organisms, and promote the successful infection of host plant cells by pathogens. Related studies on peroxidase (POX) of Fusarium graminearum have not been reported. In this study, 31 Fusarium graminearum peroxidase genes were obtained by searching the database of fungal peroxidase genes using bioinformatics methods, and were divided into 18 subfamilies by phylogenetic analysis and conserved domain analysis. Analysis of the expression pattern of the peroxidase family gene revealed that the FgPOX (Fusarium graminearum peroxidase)-13, FgPOX-17, FgPOX-16, FgPOX-19 and other genes had high expression levels in both mycelium and spore. and the FgPOX-24 and FgPOX-29 had higher expression levels in mycelium, the FgPOX-11, FgPOX-10, FgPOX-4 and FgPOX-23 had higher expression levels in spore. The expression pattern of peroxidase family gene in the process of infection was analyzed. It was found that the expression level of FgPOX-13 was significantly increased and maintained at a high level during the infection of the pathogen. In addition, genes such as FgPOX-17, FgPOX-28 and FgPOX-30 were highly expressed in the early stage of pathogen infection, and genes such as FgPOX-12, FgPOX-10 and FgPOX-23 were highly expressed in the late stage of pathogen infection. Furthermore, qRT-PCR was used to analyze the expression of Fusarium graminearum peroxidase family genes under H2O2 stress, the expression levels of FgPOX-1, FgPOX-4, FgPOX-6, FgPOX-9, FgPOX-10, FgPOX-22 and FgPOX-30 were found to be 20 times higher than that under normal conditions, and FgPOX-9, FgPOX-13, etc. with the prolongation of H2O2 stress time, the expression level was significantly enhanced, while FgPOX-1, FgPOX-21, etc. with the prolongation of H2O2 stress time, the expression level was reduced. This study clarified the number of genes, phylogenetic relationships, conserved domains of Fusarium graminearum peroxidase family, and the expression patterns of different tissues, infection processes and H2O2 stress in pathogens, which could provide basic datas for clarifying the function of Fusarium graminearum peroxidase family genes.
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