Abstract：Abstract: the RT-PCR method was established. In this test, total RNA was extracted from the fresh liver of rabbit haemorrhagic disease rabbits, a pair of primers were designed according to the sequences of RHDV published in GenBank, which can amplified a 269 bp segment. The product was sequenced and there was 95.8％～98.5％homology between the product and the relevant sequence published in GenBank. RHDV viscera distribution experiment shows that liver, kidney, spleen, blood, lung were HA positive, and all the viscera except feces were positive by RT-PCR. The sensitivity of RT-PCR for RHDV is 4×104times that of HA. All those experiments above showed the established RT-PCR method detecting RHDV has strong speciality, high sensitivity and good repetition. This RT-PCR can not only be used in RHDV clinical diagnosis and epidemiology study but also in quarantine of rabbit product ,example for meat.
收稿日期: 2006-08-04
通讯作者:
王芳
引用本文:
王芳 . 兔出血症病毒RT-PCR检测方法的建立及应用[J]. , 2007, 15(3): 0-.
. Establishment and Application of RT-PCR Test for Rabbit Hemorrhagic Disease Virus. , 2007, 15(3): 0-.
