Abstract：SRAP is a new molecular marker which aimed for the amplification of open reading frames(ORFs). It is simplicity, reliability, moderate throughput ratio and has not the specie-specific character. It had been widely used for gene tagging, germplasm collection,genetic diversity,map construction and comparing genome analysis. Factors influencing SRAP-PCR analysis were studied using three pear cultivars. A reliable,effective and reproductive PCR reaction system for detecting SRAP was developed. Each 20μL PCR reaction mixture consisted of template DNA 30ng, 2.0mmol/L of MgCl2, 200μmol/L of dNTPs, 30ng of primer and 1 unit of Taq polymerase.
收稿日期: 2006-11-20
通讯作者:
张绍铃
引用本文:
张妤艳 张绍铃. 梨SRAP-PCR反应体系的建立与优化[J]. , 2007, 15(5): 0-.
. Establishment and Optimization of SRAP-PCR in pear. , 2007, 15(5): 0-.
