Abstract：PCR primers were designed based on the conserved domain of Arabidopsis WRKY transcription factor, the full-length cDNA of WRKY transcription factor was cloned from Brasssica campestris ssp.chinensis using RT-PCR and RACE techniques. Sequence analysis indicated that BcWRKY1 gene(GenBank accession number: AY836002) consisted of 980 nucleotides(nt), and deduced amino acid sequence containing 285 amino acids. Further comparison showed its identity was 74% and 59% to AtWRKY18 gene and AtWRKY60 gene of Arabidopsis, respectively. The deduced amino acid sequence of WRKY1 gene has lower homology with other plant WRKY genes. Southern-blot analysis indicated there was a single copy in genome of B. campestris ssp.chinensis. Semi-quantitative RT-PCR demonstrated that the corresponding mRNA of WRKY1 was accumulated most abundantly 2h-8h after treatment upon 2mmol/L SA, and 6h-12h after infection by Peronospora parasitica, respectively.
收稿日期: 2007-01-30
通讯作者:
侯喜林
引用本文:
王彦华 侯喜林. 白菜WRKY转录因子cDNA全长的克隆及分析[J]. , 2007, 15(5): 0-.
. Cloning and Characterization of A full-length cDNA of WRKY transcription factor gene in Brassica campestris ssp.chinensis. , 2007, 15(5): 0-.
