Abstract：A high toxicity Bacillus thuringiensis (Bt) strain WZ-9 against larvae of Henosepilachna vigintioctomaculata was isolated from soil of Hebei province. Bipyramidal inclusions could be observed by scanning electron microscopy. SDS-PAGE analysis indicated that the strain produced a 130 kDa protein. Growth cycle of this strain was 24h, and pH of the culture varied with the strain growth. The bioassay results showed that the WZ-9 strain was highly toxic to the 2 instar larvae of H. vigintioctomaculata.. Correct mortality is 100% at 72h, and LC50 is 2.95×107cell/mL. Genotype identify showed this strain had cry7 gene. Sequence analysis showed that the encoding gene contained a ORF of 3414 bps and encoded 1138 amino acids residues. The deduced amino acid sequence is 99.65% identical to that of the reported Cry7Ab2 sequences. By Bt δ-endotoxin nomenclature committee this gene was designated as Cry7Ab3 with accession number BI 1015188 in GenBank database.
收稿日期: 2007-10-11
通讯作者:
宋萍
引用本文:
宋萍. 一株对马铃薯瓢虫幼虫有特异活性的苏云金芽孢杆菌新菌株的研究[J]. , 2008, 16(3): 0-.
Ping SONG. Study about A New Strain WZ-9 of Bacillus thuringiensis with Insecticidal Activity Against Henosepilachna vigintioctomaculata. , 2008, 16(3): 0-.
