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2025年7月30日 星期三
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干旱复水条件下诱导的糜子特异表达基因的分离与分析

林凡云;胡银岗;宋国琦;张宏;刘天明;何蓓如

西北农林科技大学农学院,国家小麦改良中心杨凌分中心,陕西省农业分子生物学重点实验室,杨凌712100
Isolation and Analysis of Genes Induced by Rehydration after Serious
Drought in Broomcorn Millet (Panicum miliaceum) by Using SSH
LIN Fan-yun;HU Yin-gang;SONG Guo-qi;ZHANG Hong;LIU Tian-ming;HE Bei-ru
College of Agronomy, Yangling Branch of China National Wheat Improvement Center, Shaanxi Key Laboratory of Molecular Biology for Agriculture,Northwest Agriculture and Forestry University, Yangling  712100,  China
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摘要 利用抑制消减杂交(suppression subtraction hybridization,SSH)技术构建了糜子(Panicum miliaceum)干旱后复水条件与正常浇水条件下基因差异表达的SSH-cDNA文库。对其中的60个阳性克隆进行了测序,在去除冗余的cDNA后,利用NCBI的BLAST软件在GenBank 中进行核酸和蛋白质同源性的比较和功能分析。核酸比对结果表明,有32个cDNA片段与已知cDNA片段具有较高的同源性,且多数与植物受到生物或非生物胁迫时的反应机制相关,同时有11条序列与小鼠肝被手术切除后再生时产生的EST有一定同源性。蛋白分析结果显示,有28个序列与已知功能蛋白序列同源性较高,这些功能蛋白涉及植物体内的信号转导、转录调控及蛋白加工等。实验结果表明糜子在干旱复水条件下可诱导一系列特异基因的表达。
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林凡云
胡银岗
宋国琦
张宏
刘天明
何蓓如
关键词 糜子干旱复水基因表达抑制消减杂交    
AbstractIn order to investigate the molecular mechanism of broomcorn millet (Panicum miliaceum) on the condition of rehydration after serious drought, a forward subtracted cDNA library was constructed between normal watering leaves of broomcorn millet                                  and rehydration ones after serious drought conditions by using SSH(suppression subtraction hybridization) technique. A total of 60 positive clones were randomly picked out from the subtracted library and sequenced, and redundancy sequences were removed after sequence alignment. Based on the result of sequence homologous comparison and function querying, 32 EST sequences were highly homologous with known EST. Most of those sequences were related to abiotic or biotic stresses in plants. Of those sequences, 11 EST sequences were homologous with mice liver ESTs of partial hepatectomy. The blast result of proteins revealed, there were 28 EST sequences were similar to known proteins. The functions of those proteins mainly involve signal transduction, transcription and protein processing. This experiment displays that a series of specific genes are induced and expressed during the rehydration stage after serious drought.
Key wordsbroomcorn millet    drought stress    rehydration    gene expression    suppression subtraction hybridization (SSH)
收稿日期: 2005-09-19     
ZTFLH:  S188  
通讯作者: 胡银岗   
引用本文:   

林凡云;胡银岗;宋国琦;张宏;刘天明;何蓓如

.

干旱复水条件下诱导的糜子特异表达基因的分离与分析

[J]. , 2006, 14(4): 537-541.
LIN Fan-yun;HU Yin-gang;SONG Guo-qi;ZHANG Hong;LIU Tian-ming;HE Bei-ru. Isolation and Analysis of Genes Induced by Rehydration after Serious
Drought in Broomcorn Millet (Panicum miliaceum) by Using SSH
. , 2006, 14(4): 537-541.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/      或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2006/V14/I4/537
 
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