Abstract：Seventy nine pairs of SSR primers were used to detect genetic diversity among 129 wheat accessions from Huang-Huai area. The results showed that 335 alleles were revealed on 79 SSR loci. Two to eight alleles were detected with the average of 4.240 alleles for each SSR locus. The B genome had the greatest variation. The PIC ( polymorphism information content) value ranged from 0.015 to 0.820 for each SSR locus. The genetic similarity varied from 0.253 to 0.909 with the average of 0.492. The cluster analysis divided the accessions into four groups and seven sub-groups, which did not accord with the districts. The cluster results could distinguish the characters of accessions and reveal relationship among them.
