Identification of MYB-related Subfamily Genes in Cajanus cajan and Functional Analysis of CcMYB-R48 Under Drought Stress
ZHANG Xiu-Qi1, DONG Bi-Ying1, SONG Zhi-Hua1, DU Ting-Ting1, LI Na1, XUE Jing-Yi1, CAO Hong-Yan1, WANG Tian-Yi1, SONG Yang-Bo3, MENG Dong1,2, YANG Qing1,2,*
1 The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China; 2 Institute of Tree Development and Gene Editing, Beijing Forestry University, Beijing 100083, China; 3 College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
Abstract：Cajanus cajan is a medicinal and dietary woody legume with rich active substances and high economic value. Abiotic and biotic stresses, including drought and diseases, are important factors in the reduction of yield and quality of C. cajan; therefore, mining key C. cajan resistance genes and analyzing their functions are the focus of C. cajan breeding research. As an important member of the MYB (v-myb avian myeloblastosis viral) family of transcription factors, MYB-related (MYB-R) subfamily genes are thought to play an important role in adversity stress. In this study, 48 CcMYB-R (CcMYB-related) family genes (CcMYB-R1~CcMYB-R48) containing conserved structural domains of the MYB-R subfamily were screened by bioinformatic methods using Arabidopsis thaliana MYB-R subfamily genes for sequence alignment in the C. cajan genome. Analysis of transcriptome data revealed that CcMYB-R48 was significantly up-regulated and highly expressed under abscisic acid (ABA), Methyl Jasmonate (MeJA) stress treatments, suggested that it might be play an important function in stress response and hormone induction in C. cajan. Cloning and analysis of the CcMYB-R48 revealed that it had a typical MYB functional domain with high homology to Arabidopsis thaliana AT1G19000 and AT1G74840. The semi-quantitative RT-PCR (SqRT-PCR) revealed that CcMYB-R48 was significantly up-regulated in expression under both 6 and 12 h of drought stress, whereas no difference was observed under 6 and 12 h of high-temperature stress, suggested its possible involved in drought resistance in C. cajan. CcMYB-R48 overexpression vector was constructed and overexpressed CcMYB-R48 in C. cajan plants were established using the C. cajan instantaneous conversion system. The results showed that the overexpression plants had lower mortality of 19.44% under drought conditions, and their electrical conductivity, malondialdehyde (MDA) content and peroxidase (POD) content were significantly lower than those of the control plants, indicated that CcMYB-R48 could enhance the drought resistance of C. cajan. This study is important to further investigate the CcMYB-R subfamily genes of C. cajan and their drought resistance mechanism and to carry out molecular resistance breeding work.
张修齐, 董碧莹, 宋治华, 杜婷婷, 李娜, 薛婧怡, 曹红燕, 王天翼, 宋洋波, 孟冬, 杨清. 木豆MYB-related亚家族基因鉴定及干旱胁迫下CcMYB-R48的功能解析[J]. 农业生物技术学报, 2022, 30(9): 1698-1712.
ZHANG Xiu-Qi, DONG Bi-Ying, SONG Zhi-Hua, DU Ting-Ting, LI Na, XUE Jing-Yi, CAO Hong-Yan, WANG Tian-Yi, SONG Yang-Bo, MENG Dong, YANG Qing. Identification of MYB-related Subfamily Genes in Cajanus cajan and Functional Analysis of CcMYB-R48 Under Drought Stress. 农业生物技术学报, 2022, 30(9): 1698-1712.
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