Transcriptome Analysis of Large-fruit Mutant During the Early Development of Fruit in 'Tonami' Apple (Malus domestica)
ZHANG Xue-Ying1, LI Zhen-Xia2, YIN Bao-Ying1, LI Zhong-Yong1, ZHANG Yuan1, XU Ji-Zhong1,*, LI Qi-Wei1, SHAO Jian-Zhu1
1 College of Horticulture, Hebei Agricultural University, Baoding 071001, China; 2 College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China
Abstract：The 'Tonami' large-fruit mutant derived from the natural mutant of 'Tonami' apple. To analysis the reason of large-fruit mutant of 'Tonami' apple (Malus domestica 'Tonami'), fruit size, cell ploidy, cellular structure and transcriptome in the early development of fruit were studied in 'Tonami' apple and its large-fruit mutant. Results showed that weight of average single fruit of 'Large-fruit Tonami' apple was significantly higher than the contrast (P<0.05), was 1.5 times of the contrast. There was no significant difference in the cell size of the fruits, but the number of cells in the 'Large-fruit Tonami' at 28 d after full bloom and at the ripeness was significantly higher than that of 'Tonami' (P<0.05). Cell ploidy were the same of 'Large-fruit Tonami' apple and contrast. Transcriptome of fruit after full bloom 14 and 28 d were compared with 'Tonami' and 'Large-fruit Tonami' apple. Fifty-one differential expressed genes (DEGs) were identified. By KEGG analysis, these unigenes were found to be implicated in plant hormone signal transduction pathway. Five genes that might be related to large-fruit mutant were selected from that pathway, these genes were mainly involwed in cytokinin signaling transduction pathway and ethylene signaling transduction. The study showed that large-fruit mutant of 'Tonami' apple might be related to the changes of plant hormones in fruit development. This study would provide basic information for further studying the cytological and molecular basis on apple large-fruit mutant.
张学英, 李振侠, 尹宝颖, 李中勇, 张媛, 徐继忠, 李琦玮, 邵建柱. ‘斗南'苹果大果芽变果实发育早期转录组分析[J]. 农业生物技术学报, 2019, 27(12): 2140-2149.
ZHANG Xue-Ying, LI Zhen-Xia, YIN Bao-Ying, LI Zhong-Yong, ZHANG Yuan, XU Ji-Zhong, LI Qi-Wei, SHAO Jian-Zhu. Transcriptome Analysis of Large-fruit Mutant During the Early Development of Fruit in 'Tonami' Apple (Malus domestica). 农业生物技术学报, 2019, 27(12): 2140-2149.
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