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2025年4月4日 星期五
农业生物技术学报  2021, Vol. 29 Issue (2): 251-257    DOI: 10.3969/j.issn.1674-7968.2021.02.005
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
红麻转录因子基因HcNAC1的分离及其在干旱胁迫下的表达模式
邓接楼1, 张高阳1,*, 张宗良1, 张超1, 徐建堂2, 祁建民2, 伍应保1, 王刚1
1 上饶师范学院 生命科学学院,上饶 334001;
2 福建农林大学 农学院,福州 350002
Isolation of HcNAC1 Transcription Factor Gene and Its Expression Patterns Under Drought Stress in Kenaf (Hibiscus cannabinus)
DENG Jie-Lou1, ZHANG Gao-Yang1,*, ZHANG Zong-Liang1, ZHANG Chao1, XU Jian-Tang2, QI Jian-Min2, WU Ying-Bao2, WANG Gang1
1 College of Life Sciences, Shangrao Normal University, Shangrao 334001, China;
2 College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
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摘要 干旱是严重影响农作物生长的非生物限制因子之一,NAC (NAM, ATAF1/2和CUC2)转录因子是植物类特有的一类转录因子,在调控植物生长发育和应对非生物胁迫过程中发挥重要作用。为研究红麻(Hibiscus cannabinus)中NAC转录因子在干旱胁迫方面的调控功能,本研究从红麻转录组数据库中分离得到一个红麻NAC类基因,命名为HcNAC1 (GenBank No. MT799841)。序列分析发现HcNAC1基因编码区包含1 044 bp,编码347个氨基酸,蛋白质分子量38.77 kD,等电点为8.78。HcNAC1具有NAM/NAC类基因家族的保守结构域,在该蛋白的N端含有160个氨基酸组成的NAC结构域,具有两个核定位信号(nuclear localization signal, NLS)序列。系统发育进化树分析表明红麻HcNAC1基因与木槿(Hibiscus syriacus)的进化关系最近。利用反转录PCR (reverse transcription PCR, RT-PCR)和qRT-PCR对该基因在不同组织部位和干旱胁迫处理24 h内的表达量进行了检测,结果表明:HcNAC1在根、茎、叶、花和果实中均有表达,叶片组织中HcNAC1随着聚乙二醇6000 (PEG6000)干旱处理的时间的延长,其表达量也逐步增加,处理12 h后,表达量达到最大值,随后逐渐下降,说明HcNAC1在响应干旱胁迫中发挥着重要的调控作用。本研究探究了HcNAC1基因表达与干旱胁迫的相互关系,为后期研究红麻NAC转录因子参与干旱逆境胁迫的分子机制提供参考依据。
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邓接楼
张高阳
张宗良
张超
徐建堂
祁建民
伍应保
王刚
关键词 红麻NAC转录因子干旱胁迫表达模式分析    
Abstract:Drought is one of the abiotic limiting factors that seriously affect the growth of crops. NAC (NAM, ATAF and CUC) transcription factor is one type of transcription factors in plants, it play an important role in regulating plant growth, development and response to abiotic stress. In order to study the regulation function of NAC transcription factor in drought stress of kenaf (Hibiscus cannabinus), a NAC like gene was isolated from kenaf transcriptome database, named HcNAC1 gene (GenBank No. MT799841). The coding sequence of HcNAC1 contained 1 044 bp, encoding 347 amino acids, protein molecular weight 38.77 kD, isoelectric point was 8.78. The HcNAC1 genes with NAM/NAC family conservative area, containing about 160 amino acid conservative in the protein N-terminal structural domain, with 2 nuclear localization signal (NLS) sequence. Phylogenetic tree analysis showed that kenaf HcNAC1 had the closest evolutionary relationship with Hibiscus syriacus. The expression level in different tissues and within 24 h of drought stress was detected by reverse transcription PCR (RT-PCR) and qRT-PCR, research results showed that HcNAC1 gene was expressed in root, stem, leaf, flower and fruit, HcNAC1 gene was expressed under PEG6000 stress and had most expression level at 12 h, and then the expression level gradually decreased. HcNAC1 played an important regulatory role in response to drought stress. The study explored the correlation between HcNAC1 expression and drought stress. and could provide a reference for further research on the molecular mechanism of HcNAC1 transcription factors involved in drought stress of kenaf.
Key wordsHibiscus cannabinus    NAC transcription factor    Drought stress    Expression pattern analysis
收稿日期: 2020-04-22      出版日期: 2021-02-01
ZTFLH:  S563.5  
基金资助:国家自然科学基金(31860396); 江西省教育厅科学技术研究项目(K6000213)
通讯作者: *gyzhang2015@163.com   
引用本文:   
邓接楼, 张高阳, 张宗良, 张超, 徐建堂, 祁建民, 伍应保, 王刚. 红麻转录因子基因HcNAC1的分离及其在干旱胁迫下的表达模式[J]. 农业生物技术学报, 2021, 29(2): 251-257.
DENG Jie-Lou, ZHANG Gao-Yang, ZHANG Zong-Liang, ZHANG Chao, XU Jian-Tang, QI Jian-Min, WU Ying-Bao, WANG Gang. Isolation of HcNAC1 Transcription Factor Gene and Its Expression Patterns Under Drought Stress in Kenaf (Hibiscus cannabinus). 农业生物技术学报, 2021, 29(2): 251-257.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2021.02.005     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2021/V29/I2/251
 
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