Effects of Different Photoperiods on the Expression of Circadian Clock Genes in Nile tilapia (Oreochromis niloticus)
FU Xu-Ze1,2, ZOU Zhi-Ying2, XIAO Wei2, ZHU Jing-Lin2, LI Da-Yu2, YU Jie2, CHEN Bing-Lin2, YANG Hong1,2,*
1 Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2 Freshwater Fisheries Research Center/Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Abstract：The circadian clock genes are the key gene to control the circadian rhythm. Light is the environmental variable that has the greatest impact on the circadian clock, changes in the photoperiod lead to changes in the circadian expression mode of the circadian clock genes, which further affect the various physiological activities such as biological living habits, development and reproduction. In order to investigate the effects of different photoperiods on the circadian rhythm of Nile tilapia (Oreochromis niloticus), the circadian expression of biological core circadian clock genes bmal1 (brain and muscle arnt-like 1), clocka (circadian locomotor output cycles kaput a), cry5 (cryptochrome 5) and per1b (period 1b) in brain, liver and eye of Nile tilapias was analyzed under 3 different photoperiods, the light (L)/dark (D) ratios were 8 h light and 16 h dark (8L∶16D), 12 h light and 12 h dark (12L∶12D), 16 h light and 8 h dark (16L∶8D), light intensity was 1 000 lx, the light start time is set to the zeitgeber time (ZT) 0 h. The result showed that bmal1, clocka, cry5, per1b displayed a circadian rhythm in brain, liver and eye under normal photoperiod condition (12L∶12D); Under 12L∶12D, the acrophase of positive regulatory genes bmal1 and clocka in liver (ZT 13.98 h, ZT 12.02 h) was earlier than that in brain (ZT 17.31 h, ZT 14.37 h), and that of negative regulatory genes cry5 and per1b in liver (ZT 7.28 h, ZT 0.92 h) was later than that in brain (ZT 4.66 h, ZT 0.76 h); Long light (16L∶8D) led to the delayed acrophase of clocka in brain, the increased amplitude of cry5 in liver; Short light (8L∶16D) caused the increased amplitude of per1b in brain, bmal1 in liver and cry5 in eye. It indicated that Nile tilapia had a biological clock system similar to other known fish. The biological rhythms of different tissues were not exactly the same. Environmental factors such as photoperiod would affect the expression of clock genes and the response of clock genes in different tissues was different. This study provides a reference basis for further elucidating the regulation mechanism of circadian clock system in Nile tilapia.
符徐泽, 邹芝英, 肖炜, 祝璟琳, 李大宇, 喻杰, 陈炳霖, 杨弘. 不同光照周期对尼罗罗非鱼生物钟基因表达的影响[J]. 农业生物技术学报, 2022, 30(3): 539-549.
FU Xu-Ze, ZOU Zhi-Ying, XIAO Wei, ZHU Jing-Lin, LI Da-Yu, YU Jie, CHEN Bing-Lin, YANG Hong. Effects of Different Photoperiods on the Expression of Circadian Clock Genes in Nile tilapia (Oreochromis niloticus). 农业生物技术学报, 2022, 30(3): 539-549.
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