Effects of Arsenic Containing Acid Mine Drainage on Soil Microbial Community Structure in Paddy Field
YANG Ze-Yan1, ZHANG Chi-Peng1,2,*, HUANG Chen-Chen1, ZHANG Kai-Xuan1, CHEN Shuang1
1 College of Resources and Environmental Engineering, Guizhou University, Guiyang 550025, China; 2 Key Laboratory of Karst Geological Resource and Environment, Ministry of Education, Guizhou University, Guiyang 550025, China
Abstract：The properties of farmland soil polluted by acid mine drainage (AMD) change greatly, which is potentially harmful to microbial growth. In order to study the impact of arsenic containing AMD on the microbial community structure in paddy field, soil samples were collected from contaminated and clean fields near one arsenic rich coal mine of Xingren city, Guizhou province. The physical and chemical properties of overlying water and soil were determined. The high-throughput sequencing technology was used to analyze the microbial community composition and diversity changes in contaminated and clean areas. The results showed that the soil in contaminated area was acidified, the pH ranged from 3.60 to 4.40, and the average contents of iron and arsenic were 114.26 g/kg and 98.70 mg/kg respectively. While the average soil pH in clean area was 5.93, and the average contents of the two elements were 106.25 g/kg and 21.63 mg/kg, respectively. Proteobacteria was the main category of microorganisms in the two areas, with an average relative abundance of 29.96%. The diversity of soil microbial community in S1 and S2 of contaminated area was significantly different from that in clean area (P<0.05). Compared with the clean area, the relative abundances of acidophilic genera Gp1, Gp2 and Gp13 increased by 3.23%, 2.88% and 1.38%, respectively. Desulfosporosinus and Desulfitobacterium were only detected in the soil of contaminated area. The abundances of Geobacter and Anaeromyxobacter in iron reducing bacteria group were relatively high. Their average proportions in contaminated area were 1.58% and 1.60%, respectively, and 1.62% and 3.02% respectively in clean area. Redundancy analysis and Spearman correlation analysis showed that soil acidification and arsenic contamination were the main environmental pressures on soil microbial community structure. The study can help to further reveal the relationship between the variation of soil environmental conditions contaminated by arsenic containing AMD and the change of microbial community structure, and provides a theoretical basis for the ecological restoration of contaminated field.
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