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2025年4月3日 星期四
  2015, Vol. 23 Issue (6): 762-771    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
尼罗罗非鱼生长激素促分泌素受体基因(GHSR)生长相关单核苷酸多态性(SNPs)位点的筛选
王春晓1,卢迈新2,高风英2,刘志刚2,曹建萌2,朱华平3,可小丽2,王淼3,叶星4,叶卫5
1. 中国水产科学研究院珠江水产研究所农业部热带亚热带水产资源利用与养殖重点实验室上海海洋大学水产与生命学院
2. 中国水产科学研究院珠江水产研究所
3. 中国水产科学研究院 珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点实验室
4. 中国水产科学研究院珠江水产研究所,中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室
5. 广东省淡水名优鱼类种苗繁育中心
Screening of Single Nucleotide Polymophisms (SNPs) Related with Growth in Growth Hormone Secretagogue Receptor Gene (GHSR) of Nile Tilapia (Oreochromis niloticus)
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摘要 本研究用直接测序法,以尼罗罗非鱼(Oreochromis niloticus)基础群体和快长群体父母本为材料进行生长激素促分泌素受体基因(growth hormone secretagogue receptor, GHSR)单核苷酸多态性(single nucleotide polymophisms, SNPs)位点的筛选;再以两群体的子代为材料,采用snapshot法对筛选到的SNPs进行基因分型,并利用一般线性模型(general linear model, GLM)对基因型与生长性状进行相关性分析。结果表明,从两个尼罗罗非鱼群体的GHSR基因中共获得S1(A-409G)、S2(G-424T)、S3(T-553A)、S4 (T-1114A)和S5(A-1168C)等5个SNPs位点,且均分布于该基因5′侧翼区。SNPs基因型与尼罗罗非鱼子代生长指标关联性分析结果表明,当S4位点为AA或AT基因型时,尼罗罗非鱼个体的体重、体长、体高、头长、尾柄长和尾柄高等生长指标均显著大于S4位点基因型为TT的个体(P<0.05),快长群体子代中S4位点AT基因型频率最高(55%),而在基础群体子代中TT基因型频率最高(75.94%)。5个SNPs位点不同基因型组成了7种双倍型(D1~7),其中D3和D5双倍型个体的体重、体长、体高、头长、尾柄高等生长指标显著高于D4和D7双倍型个体(P<0.05);而D1(体宽除外)、D2(体宽和尾柄长除外)和D6(体高除外)双倍型个体与其他双倍型个体之间在生长指标上均无显著差异(P>0.05)。快长群体子代和基础群体子代中的优势双倍型分别为D3双倍型(46.25%)和D4双倍型(35.44%)。因此,尼罗罗非鱼GHSR基因中S4位点处AA、AT基因型及双倍型D3、D5与快长性状密切相关,可作为尼罗罗非鱼分子标记辅助育种的候选标记。
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作者相关文章
王春晓
卢迈新
高风英
刘志刚
曹建萌
朱华平
可小丽
王淼
叶星
叶卫
关键词 尼罗罗非鱼生长激素促分泌素受体基因(GSHR)单核苷酸多态性位点(SNP)生长性状    
Abstract: PCR amplification and direct sequencing were used for single nucleotide polymophisms (SNPs) searching from growth hormone secretagogue receptor gene (GHSR) of the 2 populations of Nile tilapia (Oreochromis niloticus) (i.e., Fast-growing population and Base population). Snapshot method was used for genotyping of SNPs, and the correlation between genotypes of SNPs and growth traits of tilapia were analyzed using general linear model (GLM) in the offsprings of the 2 populations. Five SNPs loci (S1 (A-409G), S2 (G-424T), S3 (T-553A), S4 (T-1114A) and S5 (A-1168C)) were found in GHSR gene, and they were located in 5′ flanking region. The results showed that individuals with genotype AA or AT at S4 site had significantly higher body length, body height, head length, caudal peduncle length, caudal peduncle depth, and larger body weight than the individuals with genotype TT (P<0.05). The dominant genotype frequency of S4 site in the generation of Fast-growing population and Base population was AT (55%) and TT (75.94%), respectively. The 5 SNPs sites composed 7 diplotypes (D1~7). The body weight, body length, body height, head length and caudal peduncle depth of individuals with diplotypes D3 and D5 were significantly larger or higher than that of the individuals with diplotypes D4 and D7 (P<0.05). The growth traits of individuals with diplotypes D1 (except for body width), D2 (except for body width and the shank length) and D6 (except for body height) had no significant difference compared with individuals with the other diplotypes. The dominant diplotypes in the generation of Fast-growing population and Base population were diplotypes D3 (46.25%) and D4 (35.44%), respectively. In conclusion, the genotypes AA and AT at S4 site, diplotypes D3 and D5 of GHSR gene were closely related to fast growth rate in Nile tilapia, which can be used as candidate molecular markers for tilapia breeding.
Key wordsNile tilapia (Oreochromis niloticus)    Growth hormone secretagogue receptor gene (GHSR)    Single nucleotide polymorphisms (SNPs)    Growth traits
收稿日期: 2014-11-24      出版日期: 2015-04-13
通讯作者: 卢迈新     E-mail: mx-lu@163.com
引用本文:   
王春晓 卢迈新 高风英 刘志刚 曹建萌 朱华平 可小丽 王淼 叶星 叶卫. 尼罗罗非鱼生长激素促分泌素受体基因(GHSR)生长相关单核苷酸多态性(SNPs)位点的筛选[J]. , 2015, 23(6): 762-771.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2015/V23/I6/762
 
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