Cloning and Expression Analysis of Gonadotropic Hormone Receptors Gene in Nile Tilapia (Oreochromis niloticus)
LI Nan1,2, YU Jie2, XIAO Wei2, CHEN Bing-Lin2, ZHU Jing-Lin2, LI Da-Yu2, ZOU Zhi-Yin2, YANG Hong1,2,*
1 Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2 Freshwater Fisheries Research Center/Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Abstract：Follicle stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) belong to gonadotropic hormone receptors (GtHR) and play a regulatory role in the reproduction of fish. Nile tilapia (Oreochromis niloticus) has fast growth, short reproductive cycle and high economic value. In order to explore the relationship between GtHR and the reproductive activity of Nile tilapia, LHR and FSHR genes of Nile tilapia were cloned by using rapid amplification of cDNA ends (RACE) and homology-based cloning, and the expression changes of these two genes in ovary and testis, as well as the changes of estradiol (E2) in female and testosterone (T) in male were measured after different oxytocin injection. The results showed that the full-length of FSHR cDNA was 2 577 bp, which encoded 701 amino acids; The full-length of LHR cDNA was 3 177 bp, which encoded 693 amino acids.The results of qRT-PCR showed that FSHR and LHR genes were mainly expressed in the gonads. After the injection of oxytocin, with the change of E2 in female and T in male, FSHR showed an expression pattern of first increase then decrease and LHR showed an expression pattern of first increase, then decrease and finally increase in the gonads, and the expression level of FSHR and LHR in the injection group was significantly higher than that in the control group at multiple time points, in addition, the expression level of FSHR and LHR in the oxytocin combination injection group was higher than in the single injection group. These results indicated that FSHR and LHR might be involved in reproductive activities of Nile tilapia. Different oxytocin injections could affect the expression of FSHR and LHR genes in Nile tilapia, and the combined effect of oxytocin was better than single oxytocin. This study provides a basic information for further elucidating the physiological function and molecular mechanism of FSHR and LHR, and provids reference for reproductive regulation of Nile tilapia.
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