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2025年8月18日 星期一
农业生物技术学报  2021, Vol. 29 Issue (2): 224-239    DOI: 10.3969/j.issn.1674-7968.2021.02.003
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
马铃薯同源异形框基因家族的鉴定和表达分析
叶明辉1, 赵朋1, 牛洋1, 王冬冬1,*, 陈勤2,*
1 西北农林科技大学 农学院/旱区作物逆境生物学国家重点实验室,杨陵 712100;
2 西北农林科技大学 食品科学与工程学院,杨陵 712100
Identification and Expression Analysis of Homeobox Gene Family in Potato (Solanum tuberosum)
YE Ming-Hui1, ZHAO Peng1, NIU Yang1, WANG Dong-Dong1,*, CHEN Qin2,*
1 State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University, Yangling 712100, China;
2 College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
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摘要 同源异形框(homeobox, HB)基因在植物的生长和发育过程中起着重要的作用,许多植物的HB基因已被鉴定。但是,马铃薯(Solanum tuberosum) HB基因的完整鉴定和分类仍然未见报道。本研究在马铃薯中共鉴定了35个同源框基因,利用多种生物信息学方法分析了其理化性质、系统进化关系、染色体定位、基因和蛋白结构、潜在功能,并通过qRT-PCR技术对马铃薯HB家族基因组织特异性表达模式进行了研究。根据邻接系统发育树和结构域组成,35个马铃薯HB基因被分为6个亚家族。对外显子、内含子结构和保守模体的分析有助于进一步了解这些基因间的进化关系。马铃薯和拟南芥(Arabidopsis thaliana)以及番茄(S. lycopersicum)基因组共线性分析表明这些基因存在潜在的功能相关性。所有马铃薯HB基因都分布在12条染色体上,通过RNA测序(RNA sequencing, RNA-Seq)和qRT-PCR分析,马铃薯HB基因StBEL35 (S. tuberosum bell-type homeobox 35)、StKNOX1 (S. tuberosum KNOTTED1-like homeobox 1)和StKNOX9在贮藏器官(匍匐茎和块茎)上表现出相对较高的表达水平,且大都在成熟块茎中有更高的表达量,表明其可能在块茎发育的后期起作用。结合之前的研究, StBEL35StKNOX1StKNOX9基因可能参与赤霉素生物合成,从而影响块茎的形成,本研究有助于这些基因的功能分析,并为进一步分析马铃薯HB基因家族的分子功能提供全面的信息,为马铃薯分子育种提供理论基础。
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叶明辉
赵朋
牛洋
王冬冬
陈勤
关键词 马铃薯同源异形框(HB)表达分析块茎发育    
Abstract:Homeobox (HB) genes play an important role in diverse physiological and developmental processes of plants. The HB genes of many plants have been identified. However, the complete identification and classification of potato HB genes is still lacking. In this study, a total of 35 HB genes in the potato (Solanum tuberosum) were identified. The physicochemical properties, phylogenetic relationships, chromosome locations, gene and protein structure, and potential functions were analyzed in detail by bioinformatics. The tissue-specific expression patterns of potato HB genes were also analyzed with qRT-PCR. According to the Neighbor-joining phylogenetic tree and domain architecture, 35 potato HB genes were classified into 6 subfamilies. An analysis of the exon-intron structures and conserved motifs provided further insight into the evolutionary relationships between these genes. Synteny analysis of potato, Arabidopsis and tomato genomes provided a reference for the potential functional relevance of these HB genes. All of the potato HB genes were distributed on 12 chromosomes. Through the RNA sequencing (RNA-Seq) and qRT-PCR analyses, many potato HB genes including StBEL35 (S. tuberosum bell-type homeobox 35), StKNOX1 (S. tuberosum KNOTTED1-like homeobox 1) and StKNOX9 showed a relatively higher expression levels on storage organs (including stolon and tuber). Most of these genes showed higher expression levels in mature tubers, indicating that they may play a role in the later stages of tuber development. Combined with previous studies, these genes may be involved in gibberellins (GA) biosynthesis, thereby affecting tuber formation. This study will facilitate the functional analysis of these genes and provide comprehensive information for further analyses of the molecular functions of the potato HB gene family, and provide a theoretical basis for further molecular breeding in potato.
Key wordsPotato    Homeobox (HB)    Expression analysis    Tuber development
收稿日期: 2020-05-18      出版日期: 2021-02-01
ZTFLH:  S532  
基金资助:国家重点研发计划(2018YFD0200805); 陕西省重点研发计划(2017ZDXM-NY-004)
通讯作者: *dongdong-1025@hotmail.com;chenpeter2289@nwafu.edu.cn   
引用本文:   
叶明辉, 赵朋, 牛洋, 王冬冬, 陈勤. 马铃薯同源异形框基因家族的鉴定和表达分析[J]. 农业生物技术学报, 2021, 29(2): 224-239.
YE Ming-Hui, ZHAO Peng, NIU Yang, WANG Dong-Dong, CHEN Qin. Identification and Expression Analysis of Homeobox Gene Family in Potato (Solanum tuberosum). 农业生物技术学报, 2021, 29(2): 224-239.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2021.02.003     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2021/V29/I2/224
 
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