Cloning and Expression Analysis of 6-gingerol Biosynthesis Related Genes from Zingiber officinale
TANG Jian-Min1, QI Li-Wang2, LANG Mei-Rong1, ZHANG Wen-Lin1, LAN Jian-Bin1, LI Hong-Lei1, LI Zhe-Xin1,*
1 Chongqing Key Laboratory of Economic Plant Biotechnology/Chongqing Special Plant Industry Collaborative InnovationCenter/College of Landscape Architecture and Life Science/Institute of Special Plants, Chongqing University of Arts and Sciences, Chongqing 402160, China; 2 State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Abstract：Ginger (Zingiber officinale) has high economic value as medicinal and food resources. 6-gingerol is high important bioactive component in the pharmacological action of ginger. In the present study, a local ginger cultivar of Chongqing was used as the material. Liquid chromatograph-mass spectrometer (LC-MS) technology was used to detect the main components of pungent principle. Four cDNA sequences of 6-gingerol biosynthetic relative enzyme genes including phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate:CoA ligase (4CL) and caffeoyl-CoA-o-methyltransferase (CCoAOMT/CCOMT) (GenBank No. MN887496, MN887498, MN887497 and MN887499) were cloned and qRT-PCR was used to detect the expression of the genes in ginger rhizome at different developmental stages. Correlation analysis was performed between the expression of each gene and the 6-gingerol increase.The results showed that 6-gingerol accumulated very quickly in the 1-month ginger material after sowing, and increased slightly in the subsequent stages. PAL, C4H, 4CL and CCoAOMT genes were all highly expressed in 1~2 months ginger after sowing, suggesting a correlation between each gene and 6-gingerol synthesis. Correlation analysis showed that the expression of CCoAOMT rather than the others was significantly positively correlated with 6-gingerol synthesis (P<0.05). These results indicated that the biosynthesis of 6-gingerol existed stage specificity, and there was a positive regulatory relationship between CCoAOMT gene and 6-gingerol biosynthesis. The results of this study will lay the foundation for the further study of the function of 6-gingerol synthesis-related enzyme genes in Z. officinale.
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