Abstract：Root rot is the main disease that leads to soybean (Glycine max) yield reduction. Preliminary work screened a strain named Bacillius velezensis 3A3-15 with strong antagonistic effect on Fusarium oxysporum which is pathogenic bacteria of root rot in the soybean. To investigate the effect of biocontrol B. velezensis 3A3-15 on bacterial community structure in rhizosphere soil of potted soybean, the pots containing F. oxysporum were divide into 2 groups, the experimental group used 3A3-15 agent in the pot soil and the control group did not use 3A3-15 agent. High throughput sequencing technique was used to determine the bacterial community structure of the rhizosphere soil. Taxonomy was used to annotate the OTU (operational taxonomic units) after grouping by using the usearch61 clustering method. Alpha diversity analysis showed that Shannon index, PD (phylogenetic diversity) value, Simpson index and Chao1 index in the test group decreased, but no significance. The test group had 2 258 OTUs (operational taxonomic units) specifically, decreased by 32.74% when compared with the control group (3357). Top 10 dominant microbial bacteria at phylum level were Proteobacteria, Acidobacteria, Actinobacteria, Gemmatimonadetes, Bacteroidetes, Chloroflexi, Verrucomicrobia, Planctomycetes, TM7 and Nitrospirae. There was no significant difference in the abundance of dominant phylum between the test and control group. However, the abundance of Archaea, Tracer, Fibrobacteria and OP11 in the test group was significantly higher than that in the control group (P<0.05), and the abundance of GN02 in the test group was significantly higher than that in the control group (P<0.01). The dominant species in the 2 groups were Kaistobacter and Sphingomonas. NMDS (nonmetric multidimensional scaling) analysis showed that the difference of bacterial community in test group was smaller than that in control group. In conclusion, the addition of Bacillus velezensis 3A3-15 increased the abundance of some non-dominant bacteria and reduced the differences of bacterial community structure, but these changes were no significant in the total abundance and diversity of soil bacteria between 2 groups. The above results could provide reference data for studying on biological control mechanism and safe application of 3A3-15 biocontrol agent.
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