Genetic Analysis and Mapping of Rf-I, an Inhibitor of Fertility Restorer 3 Gene for CMS-RN in Soybean (Glycine max)
LI Yong-Kuan1,2,3, ZHANG Jing-Yong2,3, ZHAO Guo-Long2,3, LI Rong1,2,3, LIN Chun-Jing2,3, Zhao Li-Mei2,3, PENG Bao2,3,*, ZHANG Chun-Bao1,2,3,*
1 College of Agriculture, Jilin Agriculture University, Changchun 130118, China; 2 Soybean Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033, China; 3 Soybean National Engineering Research Center, Changchun 130033, China
Abstract：The "three-line" hybrid breeding method is one of the important ways to utilize crop heterosis. When the hybrid combinations were prepared, it was found that although some male-sterile lines performed well agronomically, the fertility in their F1 generation could not be fully restored. Therefore, it was speculated that there may be a gene encoding for a suppressor for the restoration of male sterility. The speculated suppressor coding gene was named as Rf-I, an inhibitor of fertility restorer gene. In this study, the RN-type cytoplasmic male sterility (CMS) soybean (Glycine max) line JLCMS89A and restorer line JLR92 were used as genetic materials. First, the genetic analysis was performed by using F1 and backcross (BC1) generation populations. Then, bulk segregant analysis (BSA) high-throughput sequencing was combined with simple sequence repeat (SSR) molecular marker technology was used to preliminarily map the Rf-I gene. The results showed that the restoration suppression trait was controlled by a single dominant gene located in the nuclear genome of the sterile line. The Rf-I was initially mapped between 38.387 and 39.890 Mb at the Chromosome 9, within the range of about 1.503 Mb. This study provides theoretical and experimental basis for subsequent research on the fine mapping of Rf-I gene and characterization of its function. In addition, the result can guide the choice of the RN-type soybean cytoplasmic male sterile lines to make hybrid soybean.
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