Abstract：Abstract: The liver proteome of Pseudosciaena crocea was analyzed through two-dimensional gel electrophoresis (2-DE) after acute low temperature stress at 8.5 ℃. Protein spots obtained from low temperature stress group were 1593± 41, and control group 1620± 37 by means of PDQuest analysis. The expressions of 16 spots expressed differentially of low temperature stress group varied significantly after challenge. 4 spots from 16 were picked out to analyze through MALDI-TOF-MS or LC-MS/MS, then searched in database. The result demonstrated that the expression of N-ethylmaleimide-sensitive fusion protein (NSF), cytokeratin-18 (CK-18) and 2-cys peroxiredoxins (2-Cys Prxs) were down-regulated remarkably, however, myosin heavy chain (MHC) up-regulated remarkably. It was concluded that the dynamic equilibrium in vivo environment of Pseudosciaena crocea was broken by low temperature stress, and various physiological reactions were induced and even death if the change was out of control.
