Abstract:AIM To study the distribution of I integron and resistance phenotype in Enterococcus isolates from different animals. Methods According to NCCLS (2007), we test 140 isolates resistant phenotype of different animal-derived using of the concentration dilution, sensitive tablet (PA) and the VITEK-AMS sensitivity test. We test the distribution of I integron by using I integrase gene PCR, and clone and sequence the representative isolates of integrase gene. Results I integrase gene positive rate was 52.14% of all and 59.52%, 52.63%, 46.67% and 46.67% of the pigs, chickens, cattle and sheep-derived. There are not significant difference (p<0.05) in Enterococcus faecalis and Enterococcus faecium. The resistant rate to amoxicillin, ampicillin, cefazolin, ciprofloxacin, clindamycin, erythromycin, gentamicin, furadantin, penicillin, rifampin, tetracycline and vancomycin were 62.14%, 60.00%, 50.71%, 77.86%, 17.14%, 69.29%, 76.43%, 20.00%, 68.57%, 80.71%, 51.43%, 0.00%. Integron-positive isolates to amoxicillin, ampicillin, gentamicin resistance compared to integron-negative isolates showed highly significant (p<0.01), to cefazolin, ciprofloxacin, clindamycin, Furadantin, rifampicin, tetracycline showed not significant (>0.05), to erythromycin and penicillin is between of the highly significant and significant (0.05>p>0.01). Conclution There are a large number of multi-drug resistant enterococci and I integron of the different animals. Suggesting that livestock production should be strengthened to monitor the resistance of enterococci to block the spread of drug-resistant strains in animals and human beings. It is indicted that I integron is very important in the dissemination of multi-drug resistant enterococci.