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2025年8月13日 星期三
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青藏高原野生老芒麦种质醇溶蛋白遗传多样性分析
鄢家俊1,白史且1,张新全2,张昌兵1,游明鸿1
1. 四川省草原科学研究院
2. 四川农业大学动物科技学院草业科学系
Genetic Diversity of Gliadin in Native Elymus sibiricus L. from Qinghai-Tibetan Plateau in China
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摘要 采用酸性聚丙烯酰胺凝胶电泳(A-PAGE) 对采集自青藏高原的54份野生老芒麦(Elymus sibiricus L.)种质进行遗传多样性分析,获得下述结果:(1)供试材料共分离出42条带纹,多态率达92.86%。4个电泳分区(α、β、γ、ω)的平均Shannon指数为0.4627,Nei-Li遗传相似系数(GS)变异范围为0.2424~0.9767,平均值为0.5822。说明供试野生老芒麦材料具有较为丰富的醇溶蛋白遗传多样性。(2)对所有材料的聚类分析和主成分分析发现,在GS值为0.562的水平上供试材料可聚成4个大类,绝大部分来自于相同或相似生态地理环境的材料聚成一类,主成分分析显示了相似的结果,即醇溶蛋白图谱类型与材料的生态地理环境具有一定的相关性。(3)基于Shannon 多样性指数估算了5个老芒麦地理类群内和类群间的遗传分化,发现类群内遗传变异占总变异的68.17%,而类群间的遗传变异占总变异的31.83 %。(4)对各地理类群基于Nei氏无偏估计的遗传一致度的聚类分析表明,各地理类群间的遗传分化与其所处的地理生态环境具有较高的相关性。
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鄢家俊
白史且
张新全
张昌兵
游明鸿
Abstract:Acid polyacrylamide gel electrophoresis (A-PAGE) was employed to detect the gliadin genetic diversity among fifty-four wild accessions of Elymus sibiricus L. collected from Qinghai-Tibetan plateau. The follow results were obtained: (1) A total of 42 bands were detected in all accessions, of which 92.86% were polymorphic. The average number of Shannon index to four electrophoretic zones (α, β, γ, ω) was 0.4627. The Nei’s genetic similarity coefficient of the tested accessions ranged from 0.2424 to 0.9767, and the average Nei’s coefficient was 0.5822. These results suggested that there was a rich genetic polymorphism among the tested wild resources of E. sibirucus. (2) 54 wild accessions can be clustered into 4 groups at GS = 0.562 level on dendrogram. The principal coordinates (PCA) reflected almost the same relationships among the studied materials as showed in cluster analysis. Moreover, the accessions from the same origin frequently clustered into one group. The findings implied that a correlation among gliadin patterns, geographical and ecological environment. (3) Genetic differentiation of between and within five eco-geographical groups of E. sibiricus is estimated by Shannon’s diversity index, which shown that 68.17% genetic variance existed within group, and 31.83% genetic variance was among groups. (4) The unweighted pairwise groups method using arithmetic average (UPGMA) cluster analysis based on Nei's unbiased measures of genetic identity was assayed for five geographical groups of E. sibiricus, which indicated that there was a significantly positive correlation between genetic differentiation and geographical habits among the five groups.
收稿日期: 2008-11-25     
通讯作者: 鄢家俊   
引用本文:   
鄢家俊1,白史且1,张新全2,张昌兵1,游明鸿1. 青藏高原野生老芒麦种质醇溶蛋白遗传多样性分析[J]. , 2009, 17(5): 891-901.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2009/V17/I5/891
 
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