Abstract：Separate Cry1Ac receptor proteins in Helicoverpa armigera Hübner midgut using two dimension electrophoresis (2D electrophoresis) method is very important for further defining kinds of receptors in H. armigera, finding new receptors and searching receptors related to Bt resistance. According to the different mode of isoelectric focus, 2D electrophoresis can be divided into two systerm: ISO-DALT (isoelectric focus-dalton weight) and IPG-DALT (immobilized pH gradient-dalton weight). In this study, combined western blotting, we compared two 2D electrophoresis instruments of ISO-DALT and IPG-DALT for the separation of Cry1Ac receptor proteins in Helicoverpa armigera. Results showed that ISO-DALT got better separation results than IPG-DALT, ISO-DALT costs less money but effect mass spectrum work, IPG-DALT is more expensive but advantage to following work.
