Abstract：Two complementary sense and antisense oligo-nucleotides, encoded Bovine Lactoferricin B Gene (LfcinB), were synthesized based on amino acids sequence of Bovine Lactoferricin B published in the antimicrobial peptide database (APD). After the sense and antisense oligo-nucleotides annealed, a cohesive BamHI site at 5’ terminus and a cohesive XhoI site at 3’ terminus.were formed. The synthetic LfcinB gene was cloned into prokaryotic expression vector pET-32a. The positive bacteria clones were amplified and cultured in terrific broth medium. Then the aimed protein was expressed with the induction of 0.1 mmol/L IPTG.. The result of SDS-PAGE indicated that LfcinB gene was strongly expressed in E. coli BL21, and the expressed recombinant protein was in form of inclusion body. Inclusion bodies of LfcinB recombinant protein were purified. The purified inclusion bodies were renatured by dialysis using TGE dialysate.
