Abstract：Both Lj-L gene and its modified version Lj-S gene from Leonurus japonicus Houtt were transformed into tobacco (Nicotiana tabacum cv. Xanthi) mediated by Agrobacterium tumefaciens. GUS and PCR identification showed that foreign gene was integrated into tobacco genome. RT-PCR analysis revealed that foreign gene could transcript normally. Analysis of resistance to pathogenic fungus (Alternaria alternata) using detatched leaves indicated that disease indexes (DI) of T0-transgenic tobacco was remarkably decreased from 100% for wild type to 27.66% for Lj-L gene and 40.77% for Lj-S gene respectively. Similar results were obtained in the in vivo analyses of resistance to pathogenic bacterium Pseudomanas solanacearum. The results suggested that the nsLTPs-like protein gene Lj-L might have great potential in generating transgenic plants with broad-spectrum resistance to both fungal and bacterial pathogens.
