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2025年5月5日 星期一
农业生物技术学报  2019, Vol. 27 Issue (1): 63-70    DOI: 10.3969/j.issn.1674-7968.2019.01.007
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
AMF摩西球囊霉调控IAA代谢促进烟草生长
赵方贵*, 董志昊*, 车永梅, 卢松冲, 张文, 刘新*
青岛农业大学 生命科学学院/山东省高校植物生物技术重点实验室,青岛 266109
AM Fungi Glomous mosseae Promote Tobacco (Nicotiana tabacum) Growth by Regulating IAA Metabolism
ZHAO Fang-Gui*, DONG Zhi-Hao*, CHE Yong-Mei, LU Song-Chong, ZHANG Wen, LIU Xin**
Key Laboratory of Plant Biotechnology in Universities of Shandong Province/Life Science College, Qingdao Agricultural University, Qingdao 266109, China
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摘要 生长素(auxin)吲哚-3-乙酸(indole-3-acetic acid, IAA)作为调节植物生长发育的重要植物激素,参与丛枝菌根真菌(arbuscular mycorrhizae fungi, AMF)与植物根系的共生过程。为探究AMF摩西球囊霉(Glomous mosseae, G.m)与植物共生过程中IAA作用的调控机制,本研究以烟草(Nicotiana tabacum)为材料,通过接种摩西球囊霉,检测摩西球囊霉与烟草共生后烟草的株高和叶面积、叶片IAA含量,以及与IAA代谢相关酶的活性和相关基因的表达量。结果表明,接种摩西球囊霉的烟草,其株高和叶面积显著高于对照组(P<0.05);烟草叶片中IAA含量增加,过氧化物酶(peroxidase, POD)和吲哚乙酸氧化酶(indole-3-acetic acid oxidase, IAAO)活性下降;同时,上调生长素合成关键酶烟草吲哚-3-乙酰胺水解酶(N. tabacum indole-3- acetamide hydrolase)基因NtAMI1、烟草生长素响应因子(N. tabacum auxin response factor)基因NtARF1和烟草生长素转录调控因子(N. tabacum Aux/IAA 9)基因NtIAA9的表达,下调烟草生长素抑制蛋白(N. tabacum auxin-repressed protein)基因NtARP1;1的表达。该研究结果为深入研究AMF促进生长的机制提供科学依据。
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赵方贵
董志昊
车永梅
卢松冲
张文
刘新
关键词 摩西球囊霉生长素(IAA)代谢生长烟草    
Abstract:Indole-3-acetic acid (IAA) as a plant hormone participates in the regulation of many plant growth and development processes and plays an important role in the symbiosis between arbuscular mycorrhiza fungi (AMF) and land plants. But the regulation mechanism of AMF infection on auxin metabolism is unclear. In this study, the effects of Glomous mosseae (G.m) infection on the tobacco (Nicotiana tabacum) shoot height, leaf area, the IAA content, activities of enzymes involved in IAA metabolism and related gene transcript levels in tobacco leaves were analyzed, aimed to elucidate the relationship between the AMF induced growth improvement and the metabolism of endogenous IAA. The results showed that G.m inoculation promoted tobacco growth displayed by improvement in increase rates in shoot height and leaf area compared with those of control (without inoculation). The IAA content, activities of peroxidase (POD) and indole-3-acetic acid oxidase (IAAO), enzymes participate in the degradation of auxin, decreased in inoculated plants. G.m infection caused up regulation in transcript levels of IAA synthetic enzyme gene (NtAMI1), IAA responsive factor genes (NtARF1) as well as auxin transcriptional regulator gene (NtIAA9), depressed the expression of auxin-repressed protein (NtARP1;1). Therefore, it can be deduced that G.m infection improved tobacco growth by promoting NtAMI1-dependent IAA production, inhibiting POD and IAAO-dependent IAA degradation, as well as increasing IAA function by up-regulating NtARF1 and NtIAA9 expression and down-regulating NtARP1;1 expression. These results provide the scientific basis lights for the deep understanding of the promoting effect of AMF on plant growth.
Key wordsGlomous mosseae    Metabolism of auxin (IAA)    Growth    Tobacco
收稿日期: 2018-06-19     
ZTFLH:  S572  
  Q945.3  
基金资助:山东省现代农业产业技术体系项目(No. SDAIT-25-04)和国家大学生创新训练项目(No. 201810435026)
通讯作者: **,liuxin6080@126.com   
引用本文:   
赵方贵, 董志昊, 车永梅, 卢松冲, 张文, 刘新. AMF摩西球囊霉调控IAA代谢促进烟草生长[J]. 农业生物技术学报, 2019, 27(1): 63-70.
ZHAO Fang-Gui, DONG Zhi-Hao, CHE Yong-Mei, LU Song-Chong, ZHANG Wen, LIU Xin. AM Fungi Glomous mosseae Promote Tobacco (Nicotiana tabacum) Growth by Regulating IAA Metabolism. 农业生物技术学报, 2019, 27(1): 63-70.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.01.007     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I1/63
 
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