AM Fungi Glomous mosseae Promote Tobacco (Nicotiana tabacum) Growth by Regulating IAA Metabolism
ZHAO Fang-Gui*, DONG Zhi-Hao*, CHE Yong-Mei, LU Song-Chong, ZHANG Wen, LIU Xin**
Key Laboratory of Plant Biotechnology in Universities of Shandong Province/Life Science College, Qingdao Agricultural University, Qingdao 266109, China
Abstract：Indole-3-acetic acid (IAA) as a plant hormone participates in the regulation of many plant growth and development processes and plays an important role in the symbiosis between arbuscular mycorrhiza fungi (AMF) and land plants. But the regulation mechanism of AMF infection on auxin metabolism is unclear. In this study, the effects of Glomous mosseae (G.m) infection on the tobacco (Nicotiana tabacum) shoot height, leaf area, the IAA content, activities of enzymes involved in IAA metabolism and related gene transcript levels in tobacco leaves were analyzed, aimed to elucidate the relationship between the AMF induced growth improvement and the metabolism of endogenous IAA. The results showed that G.m inoculation promoted tobacco growth displayed by improvement in increase rates in shoot height and leaf area compared with those of control (without inoculation). The IAA content, activities of peroxidase (POD) and indole-3-acetic acid oxidase (IAAO), enzymes participate in the degradation of auxin, decreased in inoculated plants. G.m infection caused up regulation in transcript levels of IAA synthetic enzyme gene (NtAMI1), IAA responsive factor genes (NtARF1) as well as auxin transcriptional regulator gene (NtIAA9), depressed the expression of auxin-repressed protein (NtARP1;1). Therefore, it can be deduced that G.m infection improved tobacco growth by promoting NtAMI1-dependent IAA production, inhibiting POD and IAAO-dependent IAA degradation, as well as increasing IAA function by up-regulating NtARF1 and NtIAA9 expression and down-regulating NtARP1;1 expression. These results provide the scientific basis lights for the deep understanding of the promoting effect of AMF on plant growth.
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