联系我们 加入收藏
 
年期检索 高级检索
33
2025年4月6日 星期日
  2018, Vol. 26 Issue (10): 1770-1777    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
半滑舌鳎抗哈维氏弧菌病的遗传力和育种值分析
卢昇1,李泽宇2,李仰真3,刘洋4,王杉3,杨英明3,赵玉柱3,马腾3,魏敏3,陈松林3
1. 中国海洋大学
2.
3. 中国水产科学研究院黄海水产研究所
4. 中国水产科学院 黄海水产研究所
Analyzing of Heritability and Breeding Values for Resistance Against Vibrio harveyi in Chinese Tongue Sole (Cynoglossus semilaevis)
全文: PDF (932 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 半滑舌鳎(Cynoglossus semilaevis)是我国沿海地区特有的海水经济养殖品种,在高密度工厂化养殖过程中该品种易受哈维氏弧菌(Vibrio harveyi)感染患腹水病并大量死亡,严重影响了该品种养殖产业的健康发展,本研究以本实验室2016年建立的半滑舌鳎家系为基础,从22个家系中分别选取1 999尾和1 715尾295日龄左右的幼鱼进行哈维氏弧菌人工感染实验。实验一共进行288 h,于300 h终止,2次实验感染后存活率范围分别为:0~95.45%和0~87.30%,平均存活率分别为31.17%和26.88%。成对t检验结果表明,2次感染实验中各家系感染后存活率无显著性差异(P=0.21>0.05)。本研究采用0/1(0表示死亡, 1表示存活)和每尾实验鱼在实验中的存活时间作为半滑舌鳎抗哈维氏弧菌的表型,并利用3种遗传评定模型对该性状进行分析。此外,对各家系育种值(家系中所有个体育种值均值)进行相关性分析和成对t检验以比较3种模型对该性状的估算能力。结果表明,半滑舌鳎抗哈维氏弧菌遗传力为0.17±0.05~0.36±0.20,属中低遗传力。相关性分析结果显示,3种模型估算出的各家系育种值之间的Pearson相关系数范围为0.94~0.97,呈高度正相关关系。3组家系育种值有相似的排名,成对t检验结果表明3组排名之间没有显著性差异(P>0.05)。上述结果表明,半滑舌鳎抗哈维氏弧菌属于中低遗传力性状,存在较为丰富的遗传变异,适合采用家系选育的方式对该性状进行选育。此外,3种模型对半滑舌鳎抗哈维氏弧菌遗传参数的估算能力相似。本研究在人工感染实验的基础上,利用3种遗传评定模型分析了半滑舌鳎抗哈维氏弧菌遗传参数,得到了该性状的遗传力和各家系育种值,补充了半滑舌鳎抗哈维氏弧菌遗传参数估计相关内容,为选育抗哈维氏弧菌的半滑舌鳎苗种提供了参考。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
卢昇
李泽宇
李仰真
刘洋
王杉
杨英明
赵玉柱
马腾
魏敏
陈松林
关键词 遗传力半滑舌鳎哈维氏弧菌抗病    
Abstract:Chinese tongue sole (Cynoglossus semilaevis) is an economical sea-water species which cultured in coastal areas of China. The development of the Chinese tongue sole industry was impeded due to the high frequency of disease outbreak. In this study, 1 999 and 1 715 individuals from 22 families established in 2016 were selected for challenge test with Vibrio harveyi intraperitoneal injection, respectively. The challenge test lasted 288 h, and ended at 300 h, the survival rate of two tests after injection ranged from 0 to 95.45% and 0 to 87.30%, respectively. The average survival rate of two experiments at end of test were 31.17% and 26.88%. respectively. There were no significant differences between survival rate of two experiments based on results of paired-t test (P=0.05). Two types of records, 0/1 (0 for death, and 1 for survival) and the survival time of individual until death, were used as phenotype to estimate genetic parameters for resistance against V. harveyi in C. semilaevis using three models (binary linear model (BLM), linear model with 0/1 as phenotype; binary threshold model (BTM), threshold (logit) model with 0/1 as phenotype; test-days linear model (DLM) linear model with survival time as phenotype). Besides, correlation analysis and paired-t test were conducted to test differences of predictive ability between three models based on family estimated breeding value (equals averaging of estimated breeding values (EBVs) of individuals of each family). In the present study, resistance against V. harveyi in C. semilaevis belonged to moderate-to-low heritability trait, and the heritability ranged from 0.17±0.05 to 0.36±0.20. The highest heritability was estimated by model DLM (0.36), the lowest was estimated by model BTM (0.17), the result by BLM (0.28) was between them. The Pearson correlation coefficients between family EBVs estimated by three models were 0.94 to 0.97, it revealed strong positive correlation. The three models had same ranking based on family EBVs and the ranking had no significant differences according to results of paired-t test (P=0.05). In conclusion, the resistance against V. harveyi in C. semilaevis belonged to moderate-to-low heritability trait, and there were abundant genetic variations among the selected group. Family selection was a suitable method to select promising Chinese tongue sole strains with excellent ability of V. harveyi. Three models had same ability to estimate EBVs for V. harveyi resistance in C. semilaevis. Besides, this research supplemented relevant studies on estimating genetic parameters of V. harveyi resistance in C. semilaevis, and provided reference for Chinese tongue sole disease-resistance breeding.
Key wordsHeritability    Cynoglossus semilaevis    Vibrio harveyi    Disease resistance
收稿日期: 2018-02-28      出版日期: 2018-09-26
基金资助:国家自然科学基金项目;青岛海洋科学与技术国家实验室鳌山卓越人才培养项目;国家海水鱼产业技术体系舌鳎育种岗位;山东省泰山学者攀登计划专项资助
通讯作者: 陈松林     E-mail: songwentao1983@yahoo.cn;chensl@ysfri.ac.cn
引用本文:   
卢昇 李泽宇 李仰真 刘洋 王杉 杨英明 赵玉柱 马腾 魏敏 陈松林. 半滑舌鳎抗哈维氏弧菌病的遗传力和育种值分析[J]. , 2018, 26(10): 1770-1777.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2018/V26/I10/1770
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn