Abstract：Chinese tongue sole (Cynoglossus semilaevis) is an economical sea-water species which cultured in coastal areas of China. The development of the Chinese tongue sole industry was impeded due to the high frequency of disease outbreak. In this study, 1 999 and 1 715 individuals from 22 families established in 2016 were selected for challenge test with Vibrio harveyi intraperitoneal injection, respectively. The challenge test lasted 288 h, and ended at 300 h, the survival rate of two tests after injection ranged from 0 to 95.45% and 0 to 87.30%, respectively. The average survival rate of two experiments at end of test were 31.17% and 26.88%. respectively. There were no significant differences between survival rate of two experiments based on results of paired-t test (P=0.05). Two types of records, 0/1 (0 for death, and 1 for survival) and the survival time of individual until death, were used as phenotype to estimate genetic parameters for resistance against V. harveyi in C. semilaevis using three models (binary linear model (BLM), linear model with 0/1 as phenotype; binary threshold model (BTM), threshold (logit) model with 0/1 as phenotype; test-days linear model (DLM) linear model with survival time as phenotype). Besides, correlation analysis and paired-t test were conducted to test differences of predictive ability between three models based on family estimated breeding value (equals averaging of estimated breeding values (EBVs) of individuals of each family). In the present study, resistance against V. harveyi in C. semilaevis belonged to moderate-to-low heritability trait, and the heritability ranged from 0.17±0.05 to 0.36±0.20. The highest heritability was estimated by model DLM (0.36), the lowest was estimated by model BTM (0.17), the result by BLM (0.28) was between them. The Pearson correlation coefficients between family EBVs estimated by three models were 0.94 to 0.97, it revealed strong positive correlation. The three models had same ranking based on family EBVs and the ranking had no significant differences according to results of paired-t test (P=0.05). In conclusion, the resistance against V. harveyi in C. semilaevis belonged to moderate-to-low heritability trait, and there were abundant genetic variations among the selected group. Family selection was a suitable method to select promising Chinese tongue sole strains with excellent ability of V. harveyi. Three models had same ability to estimate EBVs for V. harveyi resistance in C. semilaevis. Besides, this research supplemented relevant studies on estimating genetic parameters of V. harveyi resistance in C. semilaevis, and provided reference for Chinese tongue sole disease-resistance breeding.
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