1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China; 3 Gansu Agricultural Information Center, Lanzhou 730030, China; 4 Gansu General Station of Agro-technology Extension, Lanzhou 730020, China
Abstract：Zinc is a trace element necessary for plant growth and development. The lacking or presence of excessive zinc have severe impacts on growth of plants. Zinc transporter plays an important role in regulating zinc homeostasis in plants. In this study, the potato (Solanum tuberosum) zinc transporter gene StZnT2 (GenBank No. Xm_006358632.2) was cloned from the in vitro seedlings of potato cultivar 'Atlantic'. The results showed that the gene was 1 294 bp in length and 1 011 bp in CDS region, encoding 336 amino acids. Protein molecular weight was 36 682.78 kD. Theoretical isoelectric point was 5.63. StZnT2 was a hydrophobic transmembrane protein with a typical ZIP conserved domain. StZnT2 was located on the cell membrane. Comparative analysis of homologous proteins showed that the proteins encoded by StZnT2 gene were most similar to S. pennellii, S. lycopersicum and S. commersonii. Potato plants were stunted and root elongation was inhibited under zinc deficiency and zinc toxicity. The expression of genes under different zinc concentration gradient stress were analyzed by qPCR. The results showed that the expression level of StZnT2 was up-regulated under zinc deficiency and excessive zinc stress, and the expression level was the highest in roots. These results provide theoretical basis for further elucidating the function of StZnT2 gene in potato.
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