Abstract：miR-458b-5p mediates the Wnt/β-catenin signaling pathway to regulates the development of chicken (Gallus gallus) ovarian follicles, thus affecting the level of laying in poultry. The SNP of pri-miR-458b affects the processing of miR-458b. To explore the association of pri-miR-458b gene polymorphisms with laying trait, in this study, SNPs in pri-miR-458b gene were detected with the direct sequencing and then their correlations with the laying traits were analyzed in Jining Bairi chickens. The results showed that a total of 30 SNPs were detected in the pri-miR-458b gene, and 10 loci of them were related to laying traits. Among them, rs316393825, rs734005133, rs737338503, rs733344248, rs740622221 and rs733777709 were linkage imbalance. Their combined genotype significantly affected egg number from 42 to 49 week of age and egg number until 49 week of age. The dominant haplotype of AGCGCG could increase egg number from 42 to 49 week of age by 3.29 and egg number until 49 week of age by 8.08. Compared with the free energy of AGCGCG type, the free energy of GGCGCG type decreased by 3.3 kcal/mol, while that of GTTAAA type increased by 5.3 kcal/mol. The secondary structure stability of GGCGCG type was the highest, AGCGCG type was the second, and GTTAAA type was the lowest. This study provides valuable molecular genetic markers for auxiliary selection of laying traits in Jining Bairi chickens.
韩旭, 宋志方, 滕军, 沈国蕊, 武文惠, 王文文, 唐辉. 济宁百日鸡pri-miR-458b基因多态性及其与产蛋性状的关联分析[J]. 农业生物技术学报, 2022, 30(4): 704-715.
HAN Xu, SONG Zhi-Fang, TENG Jun, SHEN Guo-Rui, WU Wen-Hui, WANG Wen-Wen, TANG Hui. Polymorphisms of pri-miR-458b Gene and Its Association Analysis with Laying Performance in Jining Bairi Chickens (Gallus gallus). 农业生物技术学报, 2022, 30(4): 704-715.
[1] 卢立志, 李进军, 田勇, 等. 2010. 家禽单核苷酸多态性研究与应用新进展[J]. 农业生物技术学报, 18(02): 374-381. (Lu L Z, Li J J, Tian Y, et al.2010. Recent progresses on research and application of poultry single nucleotide polymorphism[J]. Journal of Agricultural Biotechnology, 18(02): 374-381.) [2] 王善禾. 2015. 鸡miR-1666前体区SNP对其成熟体生成和其靶基因调控的影响[D]. 硕士学位论文, 河南农业大学, 导师: 康相涛, pp. 16-31. (Wang S H.2015. The effect of the SNP in precursor of miR-1666 on its mature production and target genes' regulation[D]. Thesis for M. S., Henan Agricultural University, Suppervisor: Kang X T, pp. 16-31.) [3] Aendo P, Netvichian R, Viriyarampa S, et al.2018. Comparison of zinc, lead, cadmium, cobalt, manganese, iron, chromium and copper in duck eggs from three duck farm systems in Central and Western, Thailand[J]. Ecotoxicology and Environmental Safety, 161: 691-698. [4] Andreas E, Hoelker M, Neuhoff C, et al.2016. MicroRNA 17-92 cluster regulates proliferation and differentiation of bovine granulosa cells by targeting PTEN and BMPR2 genes[J]. Cell and Tissue Research, 366(1): 219-230. [5] Gariglio M, Dabbou S, Biasato I, et al.2019. Nutritional effects of the dietary inclusion of partially Defatted Hermetia illucens larva meal in Muscovy duck[J]. Journal of Animal Science and Biotechnology, 10(1): 37. [6] Goldstein D B.2001. Islands of linkage disequilibrium[J]. Nature Genetics, 29(2): 109-111. [7] Han J, Lee Y, Yeom K H, et al.2006. Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex[J]. Cell, 125(5): 887-901. [8] Harnprasopwat R, Ha D, Toyoshima T, et al.2010. Alteration of processing induced by a single nucleotide polymorphism in pri-miR-126[J]. Biochemical and Biophysical Research Communications, 399(2): 117-122. [9] Kang L, Cui X X, Zhang Y J, et al.2013. Identification of miRNAs associated with sexual maturity in chicken ovary by Illumina small RNA deep sequencing[J]. BMC Genomics, 14(1): 352. [10] Koizumi M, Morita K, Takagi M, et al.2005. SNP genotyping by allele-specific PCR using ENA primers[J]. Nucleic Acids Symposium Series, (49): 47-48. [11] Kroliczewski J, Sobolewska A, Lejnowski D, et al.2018. MicroRNA single polynucleotide polymorphism influences on microRNA biogenesis and mRNA target specificity[J]. Gene, 640: 66-72. [12] Kulibaba R A, Podstreshnyi A P.2012. Polymorphism of prolactin and growth hormone genes in chicken lines of Ukrainian selection[J]. TSitologiia I Genetika, 46(6): 75-82. [13] Li H, Sun G R, Lv S J, et al.2012. Association study of polymorphisms inside the miR-1657 seed region with chicken growth and meat traits[J]. British Poultry Science, 53(6): 770-776. [14] Li H H, Wang S B, Yan F, et al.2015. Effect of polymorphism within miRNA-1606 gene on growth and carcass traits in chicken[J]. Gene, 566(1): 8-12. [15] Shao P, Liao J Y, Guan D G, et al.2012. Drastic expression change of transposon-derived piRNA-like RNAs and microRNAs in early stages of chicken embryos implies a role in gastrulation[J]. RNA Biology, 9(2): 212-227. [16] Wang W W, Wu K L, Jia M T, et al.2018. Dynamic changes in the global MicroRNAome and transcriptome identify key nodes associated with ovarian development in chickens[J]. Frontiers in Genetics, 9: 491. [17] Wu H Z, Fan F T, Liang C Z, et al.2019. Variants of pri-miR-26a-5p polymorphisms are associated with values for chicken egg production variables and affects abundance of mature miRNA[J]. Animal Reproduction Science, 201: 93-101. [18] Zhang C, Lu J, Liu B, et al.2016. Primate-specific miR-603 is implicated in the risk and pathogenesis of Alzheimer's disease[J]. Aging (Albany NY), 8(2): 272-290. [19] Zhang J F, Ji X W, Zhou D D, et al.2013. Mir-143 is critical for the formation of primordial follicles in mice[J]. Frontiers in Bioscience-Landmark, 18: 588-597.
