Polymorphism of Estrogen Receptor α (ESR1) and β (ESR2) Gene and Its Association Analysis with Egg Quality in Wumeng Black-bone Chicken (Gallus gallus)
LI Wei-Bo1, GUO Zhi-Li2, ZHANG Yong2, WANG Tian-Song2, ZENG Shan-Shan2, OU Mao-Jun1,*, ZHANG Xi-Ben1, YE Hong-Ying1, CHEN Ze-Lin1, ZHANG Lin-Da1, ZHANG Jian-Ying1, LI Xia1
1 Bijie Institute of Animal Husbandry and Veterinary Science, Bijie 551700, China; 2 Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, College of Animal Science, Guizhou University, Guiyang 550025, China
Abstract：Wumeng black-bone chicken is a kind of local chicken breed which has been bred for more than 20 years in Bijie area of Guizhou province. Its eggs are rich in a variety of trace elements and have high research value. To explore the effect of SNP of estrogen receptor α (ESR1) and β (ESR2) genes on egg quality of Wumeng black-bone chicken, two strains of Wumeng black-bone chicken, white-shell layer hens and green-shell layer hens, were used as materials to screen SNPs of ESR1 and ESR2 genes by direct sequencing of PCR products, and their correlation with egg quality were analyzed. The results showed that SNPs were not found in the ESR1 gene. There were 4 SNPs on exon 8 and its near introns of ESR2 gene, which were g.53223010 A>T mutation in intron 7, g.53223142 G>A mutation, g.5322338 C>T mutation in exon 8, causing threonine to be a methionine, which belonged to missense mutation, and g.53223404 G>A in intron 8. The mutation of g.53223338 C>T in white-shell layer hens was low polymorphism, and the other sites were moderate polymorphism, and all sites didn't deviate from Hardy-Weinberg equilibrium. The results of linkage disequilibrium analysis showed that the SNPs g.53223010 A>T and g.53223404 G>A of ESR2 gene in 2 chicken strains were completely balanced; There were linkage disequilibrium among SNPs sites g.53223142 G>A, g.53223404 G>A and g.53223010 A>T in white-shell layer hens, but there was no linkage disequilibrium in green-shell layer hens. There was no linkage imbalance among other sites. Five identical haplotypes were detected in both strains, 8 haplotypes were detected in white-shell layer hens, and 11 haplotypes were detected in green-shell layer hens. The results of egg quality correlation analysis showed that SNPs g.53223010 A>T, g.53223404 G>A and g.5322338 C>T might be the marker sites affecting egg-type index and yolk relative quality of white-shell eggs, while SNPs g.53223142 G>A might be the marker site affecting yolk weight of green-shell laying hens. White-shell layer hens H5H5 and H1H4 diploid had significant advantages in the egg-type index and eggshell thickness (P<0.05), respectively, while green-shell layer hens H3H4 diploid had significant advantages in egg yolk weight (P<0.05). When other diploids had significant advantages in one egg quality index, this diploid had significant disadvantages in another, but they could all be used as potential genetic markers affecting egg quality. This experiment provides a new genetic marker for molecular breeding of Wumeng black bone chicken.
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