Wheat (Triticum aestivum) TaSAP2-6A Gene Marker Is Associated with Agronomic Traits in Multiple Environments
WANG Yi-Xue1,2, LI Long2, MAO Xin-Guo2, WANG Jing-Yi2, CHANG Xiao-Ping2, JING Rui-Lian2,*
1 College of Life Sciences, Shanxi Agricultural University, Taiyuan 030031, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract：Stress associated proteins (SAPs) are the A20/AN1 zinc-finger proteins which widely involved in plant response to abiotic stress. The present study investigated the correlation between TaSAP2-6A gene marker and agronomic traits in a variety of environments to provide a basis for the exploration and utilization of excellent allelic variation in wheat (Triticum aestivum). In this study, TaSAP2-6A gene (GenBank No. JQ768347.1) was isolated from wheat, with the full-length genome sequence of 3 409 bp including the upstream sequence (2 714 bp), 3' untranslated region (167 bp) and coding region (528 bp). The coding region encoded 175 amino acids consisting of an A20 domain and an AN1 domain. Using a set of 'Chinese Spring' nullisomic-tetrasomic lines, TaSAP2-6A gene was located on chromosome 6A. It was consistent with the predictions made from URGI (Unité de Recherche Génomique Info) website. The polymorphism of TaSAP2-6A gene sequence was detected by 32 wheat accessions with high diversity, and no nucleotide variation was detected in the coding region, but one InDel (insert-deletion, T/-) site was identified at 1 527 bp site of the upstream sequence. A dCAPS (derived cleaved amplified polymorphic sequence) marker was developed based on the InDel, named as InDel-1527. The genotypes of a natural population consisted of 323 accessions were detected by the marker InDel-1527 of TaSAP2-6A. Association analysis revealed that this marker was significantly associated with plant height, grain number per spike and grain yield per plant in ten environments (year×site×treatment) with well-watered, drought-stressed, heat-stressed, and drought-heat-stressed treatments. The InDel (T) was considered as the superior allelic variation to decrease plant height, and increase grain number per spike and grain yield per plant. The results provide a gene resource and its functional marker for marker-assisted selection in wheat.
[1] 常建忠, 董春林, 张正, 等. 2019. 小麦抗逆相关基因TaSAP1的5′非翻译区内含子功能分析[J]. 作物学报, 45(9): 1311-1318. (Chang J Z, Dong C L, Zhang Z, et al.2019. Function analysis of 5′untranslated region introns in drought-resistance gene TaSAP1[J]. Acta Agronomica Sinica, 45(9): 1311-1318.) [2] 李龙, 毛新国, 王景一, 等. 2018a. 小麦种质资源抗旱性鉴定评价[J]. 作物学报, 44(7): 988-999. (Li L, Mao X G, Wang J Y, et al.2018. Drought tolerance evaluation of wheat germplasm resources[J]. Acta Agronomica Sinica, 44(7): 988-999.) [3] 李龙, 彭智, 毛新国, 等. 2018b. 小麦高密度遗传图谱构建及抗旱相关生理性状的遗传解析[J]. 植物遗传资源学报, 19(3): 531-538. (Li L, Peng Z, Mao X G, et al.2018. Genetic map construction and genetic dissection of drought tolerant related physiological traits in wheat[J]. Journal of Plant Genetic Resources, 19(3): 531-538.) [4] 王彩香. 2011. 小麦抗逆相关基因TaABC1和TaSAP1/2的分离及功能分析[D]. 博士学位论文, 中国农业科学院, 导师: 景蕊莲, pp. 50-51. (Wang C X.2011. Isolation and functional analysis of stress-response genes TaABC1 and TaSAP1/2 from wheat (Triticum aestivum L.)[D]. Thesis for Ph.D., Chinese Academy of Argicultural Sciences, Supervisor: Jing R L, pp. 50-51.) [5] 王荣焕, 王天宇, 黎裕. 2007. 关联分析在作物种质资源分子评价中的应用[J]. 植物遗传资源学报, 8(3): 366-372. (Wang R H, Wang T Y, Li Y.2007. Application of association analysis in molecular evaluation of crop germplasm resources[J]. Journal of Plant Genetic Resources, 8(3): 366-372.) [6] 王瑞同, 王景一, 毛新国, 等. 2018. 小麦RING型E3泛素连接酶基因TaSDIR1-D克隆与功能分析[J]. 植物遗传资源学报, 19(5): 951-958. (Wang R T, Wang J Y, Mao X G, et al.2018. Cloning and functional analysis of a RING-type E3 ubiquitin ligase gene TaSDIR1-D in wheat[J]. Journal of Plant Genetic Resources, 19(5): 951-958.) [7] 许巧方. 2017. 普通小麦胁迫相关蛋白基因TaSAP17的克隆与功能分析[D]. 硕士学位论文, 西北农林科技大学, 导师: 奚亚军, pp. 21-22. (Xu Q F.2017. Cloning and functional analysis of stress associated protein gene TaSAP17 from wheat (Triticum aestivum L.)[D]. Thesis for M.S., Northwest A & F University, Supervisor: Xi Y J, pp. 21-22.) [8] 庄巧生. 2003. 中国小麦品种改良及系谱分析[M]. 中国农业出版社, 北京, pp. 1-5. (Zhuang Q S.2003. Chinese Wheat Improvement and Pedigree Analysis[M]. China Agriculture Press, Beijing, China, pp. 1-5.) [9] Ben Saad R, Fabre D, Mieulet D, et al.2012. Expression of the Aeluropus littoralis AlSAP gene in rice confers broad tolerance to abiotic stresses through maintenance of photosynthesis[J]. Plant Cell and Environment, 35(3): 626-643. [10] Chang J Z, Hao C Y, Chang X P, et al.2014. HapIII of TaSAP1-A1, a positively selected haplotype in wheat breeding[J]. Journal of Integrative Agriculture, 13(7): 1462-1468. [11] Chang J Z, Zhang J N, Mao X G, et al.2013. Polymorphism of TaSAP1-A1 and its association with agronomic traits in wheat[J]. Planta, 237(6): 1495-1508. [12] Charrier A, Lelievre E, Limami A M, et al.2013. Medicago truncatula stress associated protein 1 gene (MtSAP1) overexpression confers tolerance to abiotic stress and impacts proline accumulation in transgenic tobacco[J]. Journal of Plant Physiology, 170(9): 874-877. [13] Dixit A R, Dhankher O P.2011. A novel stress-associated protein 'AtSAP10' from Arabidopsis thaliana confers tolerance to nickel, manganese, zinc, and high temperature stress[J]. PLOS ONE, 6(6): e20921. [14] Gedye K R, Gonzalez-Hernandez J L, Owens V, et al. 2012. Advances towards a marker-assisted selection breeding program in prairie cordgrass, a biomass crop[J]. International Journal of Plant Genomics, 2012: 313545. [15] Goldstein D B.2001. Islands of linkage disequilibrium[J]. Nature Genetics, 29(2): 109-111. [16] Gupta P K, Rustgi S, Kulwal P L.2005. Linkage disequilibrium and association studies in higher plants: Present status and future prospects[J]. Plant Molecular Biology, 57(4): 461-485. [17] Hozain M, Abdelmageed H, Lee J, et al.2012. Expression of AtSAP5 in cotton up-regulates putative stress-responsive genes and improves the tolerance to rapidly developing water deficit and moderate heat stress[J]. Journal of Plant Physiology, 169(13): 1261-1270. [18] Jin Y, Wang M, Fu J J, et al.2007. Phylogenetic and expression analysis of ZnF-AN1 genes in plants[J]. Genomics, 90(2): 265-275. [19] Kanneganti V, Gupta A K.2008. Overexpression of OsiSAP8, a member of stress associated protein (SAP) gene family of rice confers tolerance to salt, drought and cold stress in transgenic tobacco and rice[J]. Plant Molecular Biology, 66(5): 445-462. [20] Mandaliya V B, Pandya R V, Thaker V S.2010. Single nucleotide polymorphism (SNP): A trend in genetics and genome analyses of plants[J]. General and Applied Plant Physiology, 36(3-4): 159-166. [21] Mukhopadhyay A, Vij S, Tyagi A K.2004. Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco[J]. Proceedings of the National Academy of Sciences of the USA, 101(16): 6309-6314. [22] Paux E, Sourdille P, Mackay I, et al.2012. Sequence-based marker development in wheat: Advances and applications to breeding[J]. Biotechnology Advances, 30(5): 1071-1088. [23] Stroher E, Wang X J, Roloff N, et al.2009. Redox-dependent regulation of the stress-induced zinc-finger protein SAP12 in Arabidopsis thaliana[J]. Molecular Plant, 2(2): 357-367. [24] Vij S, Tyagi A K.2006. Genome-wide analysis of the stress associated protein (SAP) gene family containing A20/AN1 zinc-finger(s) in rice and their phylogenetic relationship with Arabidopsis[J]. Molecular Genetics and Genomics, 276(6): 565-575. [25] Vij S, Tyagi A K.2008. A20/AN1 zinc-finger domain-containing proteins in plants and animals represent common elements in stress response[J]. Functional and Integrative Genomics, 8(3): 301-307. [26] Wang Y X, Xu Q F, Chang X P, et al.2018. A dCAPS marker developed from a stress associated protein gene TaSAP7-B governing grain size and plant height in wheat[J]. Journal of Integrative Agriculture, 17(2): 276-284. [27] Xu Q F, Mao X G, Wang Y X, et al.2018. A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis[J]. Journal of Integrative Agriculture, 17(3): 507-516. [28] Xuan N, Jin Y, Zhang H W, et al.2011. A putative maize zinc-finger protein gene, ZmAN13, participates in abiotic stress response[J]. Plant Cell Tissue and Organ Culture, 107(1): 101-112. [29] Zhang B, Xu W N, Liu X, et al.2017a. Functional conservation and divergence among homoeologs of TaSPL20 and TaSPL21, two SBP-Box genes governing yield-related traits in hexaploid wheat[J]. Plant Physiology, 174(2): 1177-1191. [30] Zhang N, Yin Y J, Liu X Y, et al.2017b. The E3 ligase TaSAP5 alters drought stress responses by promoting the degradation of DRIP proteins[J]. Plant Physiology, 175(4): 1878-1892.
