Association Analysis of Endogenous retrovirus (ERV) Transposon Insertion Polymorphism and Growth Traits in Intron 1 of Pig (Sus scrofa domesticus) SLC26A7 Gene
CHEN Zi-Xuan, CHI Cheng-Lin, AN Ya-Long, CHEN Cai, SONG Cheng-Yi, WANG Xiao-Yan*
College of Animal Science and Technology, Yangzhou University, Yangzhou 225002, China
Abstract：The SLC26A7 (solute carrier family 26 member 7) gene in the members of the sulfate/anion transporter gene family (SLC26) can be used as a selective chloride ion channel to regulate renal electrolyte balance and mediate thyroid hormone biosynthesis. Its mutation causes thyroid insufficiency and affects individual growth and development. In this study, comparative genomics and bioinformatics methods were used to analyze the transposon insertion of the pig (Sus scrofa domesticus) SLC26A7 gene, and the polymorphisms in different breeds and their effects on the growth characteristics of Yorkshire were detected. The results showed that SLC26A7 gene had a 280 bp ERV insertion in intron 1, and the frequency of introduction of ERV+ is higher than that of Chinese native pig breeds. The age of 100 kg body weight of ERV+/+ individuals in the Yorkshire were significantly higher than that of ERV-/- individuals (P<0.05), and the ERV+/+ individuals is significantly lower than the ERV-/-(P<0.05). Insertion of ERV in the first intron of SLC26A7 gene inhibited its promoter activity. This study confirmed that the ERV insertion in pig SLC26A7 intron 1 had a significant effect on the age of 100 kg body weight and pre-slaughter weight (P<0.05). The results provided a reference for the application of this molecular marker in pig molecular breeding.
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