Genetic Effect Analysis of IGF1R Gene on Milk Production Traits in Chinese Holstein Cow (Bos taurus)
LIU Ya-Nan1, PENG Peng1, ZHENG Wei-Jie1, HAN Bo1, YANG Chen-Dong2, LI Jian-Ming2, MA Ya-Bin2, JIANG Gui-E2, NI Jun-Qing2, SUN Dong-Xiao1,*
1 College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; 2 Hebei Provincial Station for Livestock Varieties Producing and Spreading, Shijiazhuang 050061, China
Abstract：Previous study in this group discovered that the expression level of insulin-like growth factor Ⅰ (IGF1) receptor (IGF1R) gene in liver tissue of Chinese Holstein cows (Bos taurus) during the dry period was significantly higher than that in early and peak lactation periods through transcriptome sequencing. IGF1R is a receptor tyrosine kinase that plays a critical role in the signaling of the IGF family. With directly sequencing the encoding regions and 2 000 bp of upstream and downstream flanking regions of IGF1R gene using the pooled DNA from 40 sires to verify the IGF1R gene genetic effects on lactation traits in dairy cows. The identified SNPs were genotyped in 947 Chinese Holstein cows individually by target sequencing technology. Association analysis was conducted between the SNPs and 5 milk traits using the animal model. As a result, a total of 7 SNPs were detected including 1 SNP located in exon, 5 SNPs located in intron region and 1 SNP located in 3' flanking region. Of them, single-marker association analysis results showed that 6 SNPs were found associated with milk yield, fat yield, protein yield and protein percentage significantly (P=0.0146~<0.0001). The dominant effects or allelic substitution effects of 7 SNP sites reached significantly difference (P<0.05; P<0.01). Through linkage disequilibrium (LD) analysis, 7 SNPs were linked (r2=0.85~0.99) and formed 3 blocks. Haplotype-based association analysis showed that block 1 was not significantly associated with all the 5 milk traits (P>0.05). While block 2 and block 3 were associated with milk yield, fat yield and protein yield traits (P=0.0003~<0.0001), in which H1 and H2 were dominant haplotypes that increased milk yield, fat yield and protein yield, respectively. In conclusion, IGF1R gene had significant impacts on milk yield, fat yield and protein yield traits in dairy cow, implying it could be used as a molecular marker in genomic selection.
刘亚楠, 彭朋, 郑伟杰, 韩博, 杨晨东, 李建明, 马亚宾, 蒋桂娥, 倪俊卿, 孙东晓. 中国荷斯坦牛IGF1R基因对产奶性状的遗传效应分析[J]. 农业生物技术学报, 2021, 29(8): 1518-1527.
LIU Ya-Nan, PENG Peng, ZHENG Wei-Jie, HAN Bo, YANG Chen-Dong, LI Jian-Ming, MA Ya-Bin, JIANG Gui-E, NI Jun-Qing, SUN Dong-Xiao. Genetic Effect Analysis of IGF1R Gene on Milk Production Traits in Chinese Holstein Cow (Bos taurus). 农业生物技术学报, 2021, 29(8): 1518-1527.
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