Correlation Analysis of UCP3 Gene Variation, Expression and Meat Quality Traits in Jiangkou Luobo Pig (Sus scrofa)
ZHANG Ji1, WU Xue1, QIU Gan-Yuan1, LIU Peng-Cheng1, YANG Mao-Lin2, LIU Ruo-Yu1, *
1 College of Animal Sciences, Guizhou University/Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guiyang 550025, China;
2 Science and Technology Bureau of Jiangkou County, Tongren 554400, China
Abstract：Uncoupling protein 3 (UCP3), as an important regulatory protein, is mainly involved in the regulation of the body's energy metabolism and plays an important role in lipid metabolism, fatty acid oxidation and fat deposition. It plays a decisive role in the production of ATP in oxidative phosphorylation. In order to study the relationship between UCP3 gene and pork quality traits, 11 meat quality indexes of 165 Jiangkou Luobo pigs (Sus scrofa) were determined. SNPs loci were screened by sequencing all exons of UCP3 gene amplified by mixed DNA pool. Single sample DNA was amplified and sequenced for validation and typing. At the same time, the difference of meat quality among individuals with different genotypes was analyzed. The expression of UCP3 gene of individuals with different genotypes was detected to study the relationship between meat quality and UCP3 gene expression. This study identified 6 SNPs, they were g.3127A>G, g.3205T>C, g.10439C>T, g.10441C>A, g.10466G>A and g.10479A>G/T. Population genetic parameters analysis showed that the heterozygosity except g.10479A>G/T locus was high , which indicated that the distribution of alleles in the population was relatively uniform; the number of effective alleles was high, which indicated that the degree of variation in the population was large; and the content of polymorphic information was more than 0.25, which indicated that the loci could provide some genetic information. χ2 test showed that the genotype distribution of g.3127A>G and g.3205T>C loci was in Hardy-Weinberg equilibrium, while the genotype distribution of g.10439C>T, g.10441C>A and g.10466G>A loci deviated significantly from Hardy-Weinberg equilibrium. Linkage disequilibrium analysis showed that g.3127A>G and g.3205T>C loci, g.10439C>T and g.10466G>A loci were completely linkage disequilibrium, g.10441C>A, g.10466G>A and g.10479A>G/T were strongly linkage disequilibrium. The correlation analysis between SNPs loci and meat quality showed that marbling scores of TT and GG individuals at g.10479A>G/T loci were significantly higher than those of AA and AG individuals, whereas shear stress was the opposite. Genotype-expression correlation analysis showed that g.10439C>T, g.10441C>A, g.10466G>A and g.10479A>G/T loci were significantly higher than those of others. UCP3 gene expression was negatively correlated with shear stress and positively correlated with intramuscular fat content. The results showed that g.10439C>T, g.10441C>A, g.10466G>A and g.10479A>G/T sites were important SNPs sites affecting the meat quality of Jiangkou Luobo pigs, and the increase of UCP3 gene expression was beneficial to the deposition of intramuscular fat. The results provide a reference for the screening of molecular markers that contribute to intramuscular fat deposition in Jiangkou Luobo pig.
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